Yoon K S, Chen Y P, Lovell C R, Lincoln D E, Knapp L W, Woodin S A
Biol Bull. 1994 Oct;187(2):215-222. doi: 10.2307/1542244.
Antisera against the two constituent proteins of the chloroperoxidase enzyme of the capitellid polychaete Notomastus lobatus were used to identify and localize these polypeptides by immunoblotting and indirect immunofluorescence. Immunofluorescence staining showed the enzyme to be localized primarily in tissues of the tail region of the worm. Some reactivity was also observed in the epidermis of the mid-body, but none was seen in the head region. These immunofluorescence results were supported by immunoblotting experiments, which also showed that chloroperoxidase holoenzyme is localized in the tail. Immunological results were substantiated by the distribution of enzyme activity and the in vivo products of the chloroperoxidase, 4-bromophenol, 2,4-dibromophenol, and 2,4,6-tribromophenol. Chloroperoxidase is the principal enzyme involved in the production of bromoaromatics in N. lobatus. Localization of most of this enzyme in the tail region explains the detection of high levels of bromophenols in the tail, the most exposed portion of this head-down, deposit-feeding worm. This pattern of bromoaromatic distribution is consistent with the hypothesis that the worms produce these compounds as defensive chemicals against epifaunal predators.
利用针对小头虫（Notomastus lobatus）的氯过氧化物酶的两种组成蛋白的抗血清，通过免疫印迹和间接免疫荧光来鉴定和定位这些多肽。免疫荧光染色显示该酶主要定位于虫体尾部区域的组织中。在虫体中部的表皮也观察到一些反应性，但在头部区域未观察到。免疫印迹实验支持了这些免疫荧光结果，该实验还表明氯过氧化物酶全酶定位于尾部。酶活性分布以及氯过氧化物酶的体内产物4-溴苯酚、2,4-二溴苯酚和2,4,6-三溴苯酚证实了免疫学结果。氯过氧化物酶是小头虫中参与溴代芳烃生成的主要酶。这种酶大部分定位于尾部区域，这解释了在这种头朝下的沉积取食蠕虫最暴露的尾部检测到高水平溴酚的原因。这种溴代芳烃分布模式与蠕虫产生这些化合物作为对抗表栖捕食者的防御性化学物质的假设一致。
