Two new eremophilenolides from the roots of and their anti-phytopathogenic fungal and antifeedant activities.

Two new eremophilenolides, ligushicins A () and B (), and two known compounds including -sitosterol and ursolic acid were isolated from . The structures of new compounds were established on the basis of 1D and 2D NMR data and HRESIMS data interpretation. The absolute configuration of new compounds was assigned by ECD spectroscopy, and that of ligushicins A () was confirmed by X-ray diffraction analysis. The antifungal and antifeedant activities of new compounds were evaluated against four plant pathogenic fungi and third-instar larvae of respectively. Ligushicins A () and B () exhibited potent antifungal activity against and with minimum inhibitory concentration (MIC) values ranging from 50 to 100 mg/L, while they also exhibited weak antifeedant activities.