糖尿病患者血小板中膜脂肪酸组成改变及血栓素A2生成增加。

Altered membrane fatty acid composition and increased thromboxane A2 generation in platelets from patients with diabetes.

作者信息

Prisco D, Rogasi P G, Paniccia R, Abbate R, Gensini G F, Pinto S, Vanni D, Neri Serneri G G

机构信息

Clinica Medica I, University of Florence, Italy.

出版信息

Prostaglandins Leukot Essent Fatty Acids. 1989 Jan;35(1):15-23. doi: 10.1016/0952-3278(89)90011-2.

DOI:10.1016/0952-3278(89)90011-2

PMID:2928370

Abstract

Lipid composition of platelet membranes and thromboxane A2 (TxA2) generation by platelets were investigated in 42 diabetic patients (14 with macroangiopathic complications, 10 with microangiopathy and 18 without vascular complications) and in 42 clinically healthy subjects of similar age. All subjects were on a similar dietary regimen and the adherence to diet was checked by analysis of red blood cell lipids. Platelets from all groups of diabetic patients produced increased amounts of TxA2 than platelets from controls (at least p less than 0.01) and patients with macroangiopathy (p less than 0.01). Platelet cholesterol and total platelet phospholipids were higher in patients with macroangiopathy, while the relative percentage of the different phospholipid fractions in platelet membrane and their saturated and unsaturated fatty acids were similar in the different groups. Arachidonic acid (AA) content in phosphatidylcholine (PC) was found to be significantly higher in diabetic patients than in controls (at least p less than 0.005). Moreover patients with macroangiopathy had higher AA (p less than 0.001) and lower eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) levels in PC (p less than 0.001) than the other groups of patients and controls.

摘要

对42例糖尿病患者（14例有大血管病变并发症，10例有微血管病变，18例无血管并发症）和42例年龄相仿的临床健康受试者的血小板膜脂质组成及血小板血栓素A2（TxA2）生成情况进行了研究。所有受试者饮食方案相似，通过分析红细胞脂质来检查其饮食依从性。所有糖尿病患者组的血小板产生的TxA2量均高于对照组（至少p<0.01）和有大血管病变的患者（p<0.01）。有大血管病变的患者血小板胆固醇和总血小板磷脂较高，而不同组中血小板膜不同磷脂组分及其饱和脂肪酸和不饱和脂肪酸的相对百分比相似。发现糖尿病患者磷脂酰胆碱（PC）中的花生四烯酸（AA）含量显著高于对照组（至少p<0.005）。此外，有大血管病变的患者与其他患者组及对照组相比，PC中的AA含量更高（p<0.001），二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）水平更低（p<0.001）。