Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China.
Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China; Ketuohengtong Lactobacillus Industry Institute, Qingdao Agricultural University, Qingdao, 266109, China.
Fish Shellfish Immunol. 2018 Mar;74:69-75. doi: 10.1016/j.fsi.2017.12.037. Epub 2017 Dec 25.
A feeding experiment was conducted to evaluate the effects of four strains of lactic acid bacteria (LAB) [i.e. Lactobacillus plantarum LL11 (LP), Weissella confuse LS13 (WC), Lactococcus lactis LH8 (LL) and Enterococcus faecalis LC3 (ES)] isolated from marine fish on growth, immune response and expression levels of immune-related gens in body wall of juvenile sea cucumber Apostichopus japonicus. As a result, sea cucumber had better growth performance fed supplementation of LP and ES than the control group (P < .05). Survival rate in each LAB supplementation group was significantly higher than that in control group after Vibrio splendidus challenge (P < .05). In regards to the enzyme activities, LP supplementation significantly imporved the enzyme activities of alkaline phosphatase (AKP) (P < .05), acid phosphatase (ACP) and superoxide dismutase (SOD), ES supplementation significantly imporved AKP activity (P < .05), and WC supplementation significantly imporved ACP activity (P < .05). However, lysozyme (LSZ) activity was not significantly changed in the four LAB supplementation treatments (P > .05). For the gene expression levels, different expression patterns were observed among four groups, heat shock proteins (HSP60, HSP70 and HSP90) and caspase-2 showed dramatic up-regulation at 30 d while NF-kappa-B transcription factor p65 was down-regulated at 15 d and up-regulated at 30 d, and nitric oxide synthase was down-regulated at both timepoints in almost all the four groups. In conclusion, the four LAB strains screened from marine fish supplemented in diets indicated positive effects on immune response for A. japonicus, especially, the L. plantarum LL11 and E. faecalis LC3 indicated better growth performance.
进行了一项饲养实验，以评估从海洋鱼类中分离的四种乳酸菌（LAB）[即植物乳杆菌 LL11（LP）、魏斯氏菌混淆 LS13（WC）、乳球菌乳脂亚种 LH8（LL）和粪肠球菌 LC3（ES）]对幼年海参刺参生长、免疫反应和体壁免疫相关基因表达的影响。结果表明，与对照组相比，补充 LP 和 ES 的海参生长性能更好（P<0.05）。在灿烂弧菌攻毒后，每个 LAB 补充组的存活率均显著高于对照组（P<0.05）。就酶活性而言，LP 补充显著提高碱性磷酸酶（AKP）（P<0.05）、酸性磷酸酶（ACP）和超氧化物歧化酶（SOD）的酶活性，ES 补充显著提高 AKP 活性（P<0.05），WC 补充显著提高 ACP 活性（P<0.05）。然而，四种 LAB 补充处理对溶菌酶（LSZ）活性没有显著影响（P>0.05）。对于基因表达水平，四个组之间观察到不同的表达模式，热休克蛋白（HSP60、HSP70 和 HSP90）和半胱天冬酶-2 在 30d 时表现出明显的上调，而 NF-κB 转录因子 p65 在 15d 时下调，在 30d 时上调，而一氧化氮合酶在四个组的几乎所有时间点都下调。总之，从海洋鱼类中筛选出的四种 LAB 菌株添加到饮食中对刺参的免疫反应有积极影响，特别是植物乳杆菌 LL11 和粪肠球菌 LC3 表现出更好的生长性能。
