Zhao Hua, Liu Aihua, Shen Longqiang, Xu Cuiping, Zhu Ziwei, Yang Jiaru, Han Xinling, Bao Fukai, Yang Weimin
Department of Microbiology and Immunology, Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan 650500, P.R. China.
Exp Ther Med. 2017 Dec;14(6):5974-5980. doi: 10.3892/etm.2017.5300. Epub 2017 Oct 13.
The plant is distributed primarily in India, Thailand, China, Egypt and Brazil and has a history of use in the treatment of multiple diseases. Isoforskolin (ISOF) is the principle active component of native to China and has previously been studied for its biological effects. The aim of the present study was to evaluate the effect of ISOF on the proinflammatory responses induced by recombinant basic membrane protein A (rBmpA). In experiments, the proinflammatory effects of rBmpA and the anti-inflammatory function of ISOF were evaluated in murine macrophages, human macrophages and dendritic cells by detecting the transcription and expression of tumor necrosis factor (TNF)-α and interleukin (IL)-6. In experiments, mean arthritis index and X-ray and histopathological examinations were used to verify the role of ISOF in experimental Lyme arthritis in mice. The results indicated that rBmpA, which induced the transcription and expression of TNF-α and IL-6, activated proinflammatory responses in murine macrophages, human macrophages and dendritic cells. In turn, ISOF downregulated the transcription and expression of TNF-α and IL-6 induced by rBmpA. Additionally, the experiments demonstrated that ISOF could also inhibit the symptoms of experimental Lyme arthritis. These results suggest that ISOF may have a potential application as an anti-inflammatory agent for the treatment of Lyme arthritis.
该植物主要分布在印度、泰国、中国、埃及和巴西，并且有着用于治疗多种疾病的历史。异佛司可林（ISOF）是中国本土植物的主要活性成分，此前已对其生物学效应进行过研究。本研究的目的是评估ISOF对重组碱性膜蛋白A（rBmpA）诱导的促炎反应的影响。在体外实验中，通过检测肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6的转录和表达，评估rBmpA在小鼠巨噬细胞、人巨噬细胞和树突状细胞中的促炎作用以及ISOF的抗炎功能。在体内实验中，采用平均关节炎指数、X射线和组织病理学检查来验证ISOF在小鼠实验性莱姆关节炎中的作用。结果表明，rBmpA诱导TNF-α和IL-6的转录和表达，激活了小鼠巨噬细胞、人巨噬细胞和树突状细胞中的促炎反应。相应地，ISOF下调了rBmpA诱导的TNF-α和IL-6的转录和表达。此外，体内实验表明ISOF还可以抑制实验性莱姆关节炎的症状。这些结果表明，ISOF作为一种抗炎剂在治疗莱姆关节炎方面可能具有潜在应用价值。
