a Nanyang Environment & Water Research Institute (Environmental Chemistry and Materials Centre), Interdisciplinary Graduate School , Nanyang Technological University , Singapore , Singapore.
b School of Materials Science and Engineering , Nanyang Technological University , Singapore , Singapore.
J Biomater Sci Polym Ed. 2018 May-Jun;29(7-9):1081-1093. doi: 10.1080/09205063.2017.1421345. Epub 2018 Jan 3.
The intrinsically high cysteine content in human hair keratins and keratin associated proteins confer hair its outstanding mechanical strength through the formation of strong intermolecular disulfide bonds. In addition, these proteins offer the potential to be exploited as potent antioxidants. This report presents our findings on the antioxidant effects of human hair protein extracts and their consequent protective role against oxidative stress in human dermal fibroblast (HDF) cultures. Protein extracts were obtained from human hair using sodium sulfide as the reducing agent, and characterized using SDS-PAGE, Western blotting, MALDI-ToF mass spectrometry and amino acid analysis. Cysteine was found to account for 11.2 mol % in the extracted fractions. By measuring 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging activity, the hair protein fractions were shown to possess significant antioxidant ability (IC = 16.22 μM). As a supplement in cell culture media, the extracts protected HDFs from HO induced oxidative stress, which was demonstrated by the maintenance of cell viability and reduced reactive oxygen species production. Besides offering mechanical support as a scaffolding material, the unique antioxidizing ability of human hair protein extracts may also be exploited in biomedical applications.
头发角蛋白和角蛋白相关蛋白中的高半胱氨酸含量赋予了头发卓越的机械强度，这是通过形成强的分子间二硫键实现的。此外，这些蛋白质具有被开发为有效抗氧化剂的潜力。本报告介绍了我们关于人发蛋白提取物的抗氧化作用及其对人真皮成纤维细胞（HDF）培养物氧化应激的保护作用的研究结果。使用亚硫酸钠作为还原剂从人发中提取蛋白质提取物，并使用 SDS-PAGE、Western blot、MALDI-ToF 质谱和氨基酸分析进行表征。发现半胱氨酸在提取的级分中占 11.2 mol%。通过测量 2,2-二苯基-1-苦基肼水合物（DPPH）自由基清除活性，表明毛发蛋白具有显著的抗氧化能力（IC=16.22 μM）。作为细胞培养介质中的补充物，提取物保护 HDF 免受 HO 诱导的氧化应激，这通过维持细胞活力和减少活性氧的产生来证明。除了作为支架材料提供机械支撑外，人发蛋白提取物的独特抗氧化能力也可能被用于生物医学应用。
