Aix-Marseille University, CNRS, Architecture et Fonction des Macromolécules Biologiques (AFMB), UMR 7257 Marseille, France.
Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Istituto di Biologia e Patologia Molecolari del CNR, Dipartimento di Scienze Biochimiche "A. Rossi Fanelli", Sapienza Università di Roma, 00185 Rome, Italy.
Int J Mol Sci. 2017 Dec 29;19(1):91. doi: 10.3390/ijms19010091.
InSiDDe (In Silico Disorder Design) is a program for the in silico design of intrinsically disordered proteins of desired length and disorder probability. The latter is assessed using IUPred and spans values ranging from 0.55 to 0.95 with 0.05 increments. One to ten artificial sequences per query, each made of 50 to 200 residues, can be generated by InSiDDe. We describe the rationale used to set up InSiDDe and show that an artificial sequence of 100 residues with an IUPred score of 0.6 designed by InSiDDe could be recombinantly expressed in at high levels without degradation when fused to a natural molecular recognition element (MoRE). In addition, the artificial fusion protein exhibited the expected behavior in terms of binding modulation of the specific partner recognized by the MoRE. To the best of our knowledge, InSiDDe is the first publicly available software for the design of intrinsically disordered protein (IDP) sequences. InSiDDE is publicly available online.
InSiDDe（计算机内疾病设计）是一个用于设计具有所需长度和无序概率的内在无序蛋白质的程序。无序概率使用 IUPred 进行评估，范围从 0.55 到 0.95，增量为 0.05。InSiDDe 可以生成每个查询的一到十个人工序列，每个序列由 50 到 200 个残基组成。我们描述了设置 InSiDDe 所使用的原理，并表明通过 InSiDDe 设计的具有 IUPred 评分为 0.6 的 100 个残基的人工序列在与天然分子识别元件 (MoRE) 融合时可以在 中以高水平表达而不降解。此外，该人工融合蛋白在与 MoRE 识别的特定伴侣的结合调节方面表现出预期的行为。据我们所知，InSiDDe 是第一个可用于设计内在无序蛋白 (IDP) 序列的公开可用软件。InSiDDE 可在线公开获取。
