James J F, Chu M C, Lee L, Peck S A, McKissick C, Sullivan H, Frogner K, Melish M
Department of Tropical Medicine, John A. Burns School of Medicine, University of Hawaii, Honolulu.
Rev Infect Dis. 1989 Jan-Feb;11 Suppl 1:S157-66. doi: 10.1093/clinids/11.supplement_1.s157.
Batch and chemostat culture of Staphylococcus aureus strain S411 was conducted in an investigation of the role of Mg++ in the control of production of toxic shock syndrome toxin 1 (TSST-1). Under both growth conditions, Mg++ influenced bacterial growth, and TSST-1 production was correlated with bacterial growth. The specific activity of TSST-1 (ng/mg yield, ng/mg total protein) increased with increasing concentrations of Mg++ and was maximal at physiologic levels of Mg++. No production of TSST-1 was observed under anaerobic conditions. In chemostat cultures in which valine nutrient limitation was used with various levels of tryptophan in the chemically defined medium, tryptophan concentration controlled the production of TSST-1 by strain S411, regardless of the concentration of Mg++.
对金黄色葡萄球菌菌株S411进行分批培养和恒化器培养，以研究Mg++在控制毒性休克综合征毒素1（TSST-1）产生中的作用。在两种生长条件下，Mg++均影响细菌生长，且TSST-1的产生与细菌生长相关。TSST-1的比活性（ng/毫克产量，ng/毫克总蛋白）随Mg++浓度的增加而增加，在生理水平的Mg++时达到最大值。在厌氧条件下未观察到TSST-1的产生。在化学限定培养基中使用缬氨酸营养限制和不同水平色氨酸的恒化器培养中，无论Mg++浓度如何，色氨酸浓度都控制着菌株S411中TSST-1的产生。
