Dickgiesser N, Wallach U
Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Mar;263(4):572-6.
The influence of magnesium on the production of toxic shock syndrome toxin-1 (TSST-1) by Staphylococcus aureus was examined. As media we used: Standard-I-Nutrient Broth, Todd Hewitt Broth, Mueller-Hinton Broth, Isosensitest Broth and a chemically defined liquid medium. The magnesium content of these media was determined using flame photometry and was subsequently changed using magnesium sulfate to the magnesium concentrations as shown in table 1. In each of these media the TSST-1 positive S. aureus strains MN8 and T 40 were grown at 37 degrees C, 18 h, vigorously shaken. Then the colony forming units (cfu) were determined. Toxin assays were performed by immunodiffusion after concentrating the culture fluids 100-fold using ethanol precipitation. Concentrations of toxin per milliliter were determined by comparison with standard toxin preparations using hyperimmune-TSST-1 antisera as described. The cfu and the amount of TSST-1 produced are shown in table 1. No link could be demonstrated between TSST-1 production and magnesium concentration of the media used. Our results are in agreement with the ones published by Schlievert and disagree with the results from Mills.
研究了镁对金黄色葡萄球菌产生中毒性休克综合征毒素-1（TSST-1）的影响。我们使用的培养基有：标准I营养肉汤、托德-休伊特肉汤、穆勒-欣顿肉汤、异感试验肉汤和一种化学成分确定的液体培养基。使用火焰光度法测定这些培养基中的镁含量，随后用硫酸镁将其镁浓度改变为表1所示的浓度。在这些培养基中，TSST-1阳性的金黄色葡萄球菌菌株MN8和T40在37℃下剧烈振荡培养18小时。然后测定菌落形成单位（cfu）。使用乙醇沉淀将培养液浓缩100倍后，通过免疫扩散进行毒素测定。如所述，使用超免疫TSST-1抗血清与标准毒素制剂比较，测定每毫升毒素的浓度。cfu和TSST-1的产生量见表1。在所使用的培养基中，未发现TSST-1产生与镁浓度之间存在关联。我们的结果与施利弗特发表的结果一致，与米尔斯的结果不同。
