Kang Sungmin, Bruyns-Haylett Michael, Hayashi Yurie, Zheng Ying
School of Biological Sciences, Whiteknights, University of Reading.
Department of Bioengineering, Imperial College.
J Vis Exp. 2017 Nov 30(129):56447. doi: 10.3791/56447.
Although electroencephalography (EEG) is widely used as a non-invasive technique for recording neural activities of the brain, our understanding of the neurogenesis of EEG is still very limited. Local field potentials (LFPs) recorded via a multi-laminar microelectrode can provide a more detailed account of simultaneous neural activity across different cortical layers in the neocortex, but the technique is invasive. Combining EEG and LFP measurements in a pre-clinical model can greatly enhance understanding of the neural mechanisms involved in the generation of EEG signals, and facilitate the derivation of a more realistic and biologically accurate mathematical model of EEG. A simple procedure for acquiring concurrent and co-localized EEG and multi-laminar LFP signals in the anesthetized rodent is presented here. We also investigated whether EEG signals were significantly affected by a burr hole drilled in the skull for the insertion of a microelectrode. Our results suggest that the burr hole has a negligible impact on EEG recordings.
尽管脑电图（EEG）作为一种记录大脑神经活动的非侵入性技术被广泛应用，但我们对EEG神经发生的理解仍然非常有限。通过多层微电极记录的局部场电位（LFP）可以更详细地描述新皮层不同皮质层同时发生的神经活动，但该技术具有侵入性。在临床前模型中结合EEG和LFP测量可以极大地增强对EEG信号产生所涉及神经机制的理解，并有助于推导更现实、生物学上更准确的EEG数学模型。本文介绍了一种在麻醉啮齿动物中获取同步且共定位的EEG和多层LFP信号的简单程序。我们还研究了为插入微电极在颅骨上钻的小孔是否会对EEG信号产生显著影响。我们的结果表明，该小孔对EEG记录的影响可忽略不计。
