Krasnoselskiy N V, Simonova L I, Gertman V Z, Pushkar E S
SI «Grygoriev Institute for Medical Radiology, National Academy of Medical Sciences of Ukraine»,Kharkiv, Pushkinska street, 82, 61024, Ukraine.
Probl Radiac Med Radiobiol. 2017 Dec;22:202-207.
To reveal the effect of photodynamic therapy on the healing of experimental radiation skin ulcers infect ed with Pseudomonas aeruginosa.
The infected radionuclide was modeled by local X ray irradiation of the rat's hip skin at a dose of 85.0 Gy, followed by the application of Pseudomonas aeruginosa bacterial suspension to the surface of the ulcer. Photodynamic therapy (CTD) was performed with the help of a photon apparatus «Barva LED/630» and a photosen sitizer of methylene blue. The effect of PDT on the rate and quality of healing of an infected radionuclide was deter mined by comparing the histological and morphometric study of skin from the region of ulcer in animals from the control and treated groups of PDT.
The presence of Pseudomonas aeruginosa in the radiation sickness caused an increase in the area of the wound cavity compared with the non infected radionuclide and to a significant inhibition of its healing processes. The addition of the infection factor reduced the activity of the reparative processes in the radiation sick ness by 20 % compared with the uninfected. The effect of photodynamic therapy contributed to complete healing on average one month earlier than in control groups.
揭示光动力疗法对感染铜绿假单胞菌的实验性放射性皮肤溃疡愈合的影响。
通过对大鼠臀部皮肤进行局部X射线照射，剂量为85.0 Gy，模拟感染放射性核素，随后将铜绿假单胞菌菌悬液涂抹于溃疡表面。借助“Barva LED/630”光子仪器和亚甲蓝光敏剂进行光动力疗法（CTD）。通过比较光动力疗法对照组和治疗组动物溃疡区域皮肤的组织学和形态学研究，确定光动力疗法对感染放射性核素愈合速度和质量的影响。
与未感染放射性核素相比，放射性疾病中铜绿假单胞菌的存在导致伤口腔面积增加，并显著抑制其愈合过程。与未感染相比，感染因素的加入使放射性疾病中修复过程的活性降低了20%。光动力疗法的效果有助于平均比对照组提前一个月实现完全愈合。
