Chumak A A, Talko V V, Atamanyuk N P, Derevyanko L P, Rodionova N K, Kosyakova G V, Mehed A F, Goridko T M, Berdyshev A G, Gula N M
State Institution «National Research Center for Radiation Medicine NAMS of Ukraine», Melnykova str., 53, Kyiv 04050, Ukraine.
Palladin Institute of Biochemistry, NAS of Ukraine, Leontovycha str., 9, Kyiv, 01030, Ukraine.
Probl Radiac Med Radiobiol. 2017 Dec;22:270-281.
to explore possible transgeneration effects in the rats offspring of the first generation of parents subject ed to the combined effects of N stearoylethanolamine (NSE) and external exposure.Materials and metods. In the first generation rats of both sexes born to parents who have experienced the combined influence of ionizing radiation at a dose of 2.0 Gy and NSE a daily dose of 50.0 mg/kg, administered before or after exposure indicators of pro and antioxidant systems (the concentration of TBA reactive products, catalase and glu tathione peroxidase activity in plasma) were defined, concentrations of sex hormones testosterone and estradiol and nitrite anion were studied.
Irradiation of parents caused a three fold reduction of testosterone in the blood plasma of males progeny, increased activity of catalase in plasma of female offsprings, as well as significantly increased the concentration of protein in the offsprings' blood plasma of both sexes. Introduction of NSE to parents before exposure caused the acti vation of lipid peroxidation in plasma of both sexes offsprings' against the background of a trustworthy decrease in activity of antioxidant enzymes (catalase and glutathione peroxidase), however, prevented a sharp reduction of testosterone content in the blood plasma of males offsprings, conditioned by the influence of radiation on the body of their parents. NSE introduction to parents after exposure caused no significant violations of pro/ antioxidant bal ance in the body of both sexes progeny, but did not eliminate the negative impact of parental exposure to testos terone levels in male offsprings.
The transgeneration impact of NSE is manifested by radio sensitizing properties in the first generation offsprings in case of application to parents before irradiation.
探讨第一代亲代大鼠在受到N-硬脂酰乙醇胺（NSE）与外照射联合作用后，对其后代可能产生的跨代效应。材料与方法。第一代大鼠的双亲接受了2.0 Gy剂量的电离辐射与每日50.0 mg/kg的NSE联合作用，在其后代出生后，测定亲代和抗氧化系统的指标（血浆中TBA反应产物的浓度、过氧化氢酶和谷胱甘肽过氧化物酶的活性），研究性激素睾酮和雌二醇以及亚硝酸根阴离子的浓度。
亲代受到辐射导致雄性后代血浆中睾酮含量降低了三倍，雌性后代血浆中过氧化氢酶活性增加，并且两性后代血浆中的蛋白质浓度均显著增加。在亲代照射前给予NSE，在抗氧化酶（过氧化氢酶和谷胱甘肽过氧化物酶）活性可靠降低的背景下，导致两性后代血浆中脂质过氧化激活，然而，预防了辐射对其亲代机体影响所导致的雄性后代血浆中睾酮含量的急剧降低。在亲代照射后给予NSE，未导致两性后代机体中促氧化/抗氧化平衡出现显著紊乱，但并未消除亲代照射对雄性后代睾酮水平的负面影响。
在亲代照射前应用NSE时，NSE的跨代影响在第一代后代中表现为放射增敏特性。
