Cancer Biomark. 2018 Feb 14;21(3):557-563. doi: 10.3233/CBM-170431.
The aim in this study was to explore the role of long non-coding RNA GHET1 in development of non small cell lung cancer (NSCLC).
LncRNA GHET1 expression levels were analyzed by qRT-PCR in tumor tissues and adjacent normal tissues in NSCLC. Measuring the cell proliferation and invasion abilities by CCK8, cell colony formation and transwell invasion assays. Relative protein expression was analyzed by western blot assays.
Expression of lncRNA GHET1 was notably higher in NSCLC tissues compared with adjacent normal tissues by using qRT-PCR analyses. Higher lncRNA GHET1 expression associated with lymph node metastasis, TNM stage and showed poor outcome in NSCLC patients. Knockdown of lncRNA GHET1 suppressed cell proliferation and invasion capacity and Epithelial-Mesenchymal Transition (EMT) phenomenon of NSCLC cells. Moreover, we demonstrated that knockdown of lncRNA GHET1 suppresses LATS1/YAP pathway signaling pathway by downregulating YAP1 expression in NSCLC cells.
GHET1 predicted a poor outcome and acted as a tumor-promoting gene in NSCLC. Thus, inhibition of GHET1 may be a potential target of NSCLC treatment.
本研究旨在探讨长链非编码 RNA GHET1 在非小细胞肺癌（NSCLC）发展中的作用。
通过 qRT-PCR 分析 NSCLC 肿瘤组织和相邻正常组织中的 lncRNA GHET1 表达水平。通过 CCK8 法、细胞集落形成和 Transwell 侵袭实验测量细胞增殖和侵袭能力。通过 Western blot 分析相对蛋白表达。
通过 qRT-PCR 分析，发现 lncRNA GHET1 在 NSCLC 组织中的表达明显高于相邻正常组织。较高的 lncRNA GHET1 表达与淋巴结转移、TNM 分期相关，并提示 NSCLC 患者预后不良。敲低 lncRNA GHET1 抑制 NSCLC 细胞的增殖和侵袭能力以及上皮间质转化（EMT）现象。此外，我们证明通过下调 YAP1 表达，敲低 lncRNA GHET1 抑制 NSCLC 细胞中的 LATS1/YAP 通路信号通路。
GHET1 预测预后不良，并在 NSCLC 中作为促肿瘤基因发挥作用。因此，抑制 GHET1 可能是 NSCLC 治疗的潜在靶点。
