同源域转录因子 2 在结肠癌细胞中的意义。

The significance of homeodomain transcription factor 2 in colon cancer cells.

机构信息

Department of Oncology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road Middle, Shanghai, 200072, People's Republic of China.

Department of Interventional Oncology, Shanghai DaHua Hospital, Shanghai, 200072, People's Republic of China.

出版信息

Biomed Eng Online. 2021 Aug 9;20(1):81. doi: 10.1186/s12938-021-00912-5.

BACKGROUND

Colon cancer is a serious malignant tumor. It has been reported that paired-like homeodomain transcription factor 2 (PITX2) can promote the progression of several types of cancer via regulating the Wnt/β-catenin pathway. It has also been demonstrated that high levels of long non-coding RNA (lncRNA) gastric carcinoma high expressed transcript 1 (GHET1) can also promote the development of cervical cancer via activating the Wnt/β-catenin pathway. However, whether PITX2 can affect the development of colon cancer via regulating the expression of lncRNA GHET1 remains unclear.

RESULTS

The results demonstrated that PITX2 knockdown attenuated the proliferation, migration and invasion abilities of colon cancer cells. Additionally, PITX2 promoted the expression of lncRNA GHET1 via binding to its promoter. Overexpression of lncRNA GHET1 induced the expression of Wnt/β-catenin signaling-related proteins, cyclin D1, c-Myc and MMP-7. Furthermore, lncRNA GHET1 overexpression abrogated the PITX2 silencing-mediated decreased proliferation, migration and invasion abilities of colon cancer cells.

CONCLUSION

The findings of the present study suggested that PITX2 could enhance the proliferation, migration and invasion abilities of colon cancer cells via upregulating lncRNA GHET1 and activating the Wnt/β-catenin pathway.

背景

结肠癌是一种严重的恶性肿瘤。有报道称，配对同源盒转录因子 2（PITX2）可以通过调节 Wnt/β-catenin 通路促进多种类型癌症的进展。此外，长链非编码 RNA（lncRNA）胃腺癌高表达转录本 1（GHET1）水平升高也可以通过激活 Wnt/β-catenin 通路促进宫颈癌的发展。然而，PITX2 是否可以通过调节 lncRNA GHET1 的表达来影响结肠癌的发展尚不清楚。

结果

研究结果表明，PITX2 敲低可减弱结肠癌细胞的增殖、迁移和侵袭能力。此外，PITX2 通过结合其启动子促进 lncRNA GHET1 的表达。lncRNA GHET1 的过表达诱导 Wnt/β-catenin 信号通路相关蛋白、细胞周期蛋白 D1、c-Myc 和 MMP-7 的表达。此外，lncRNA GHET1 的过表达可消除 PITX2 沉默介导的结肠癌细胞增殖、迁移和侵袭能力下降。