血清和外泌体中的 lncRNA-HEIH 作为 HCV 相关肝细胞癌的潜在生物标志物。
lncRNA-HEIH in serum and exosomes as a potential biomarker in the HCV-related hepatocellular carcinoma.
机构信息
Department of Infectious Diseases, First Hospital Affiliated to Suzhou University, Suzhou, Jiangsu 215006, China.
Department of Intervention and Radiotherapy, Huzhou Central Hospital, Huzhou, Zhejiang 313000, China.
出版信息
Cancer Biomark. 2018 Feb 14;21(3):651-659. doi: 10.3233/CBM-170727.
BACKGROUND
Chronic hepatitis C (CHC) is a contagious liver disease that results from infection with the hepatitis C virus (HCV). The most serious consequence of CHC is HCV-related hepatocellular carcinoma (HCC).
OBJECTIVE
To illustrate the clinical significance of lncRNA HEIH expression in serum and exosomes in the development of HCV-related HCC.
METHODS
Thirty-five CHC, twenty-two HCV-induced cirrhosis and ten HCV-related HCC patients in Huzhou Central Hospital from January 2016 to September 2016 were recruited in the present study. Basic patient information, clinical serological indicators, and clinical imaging data were investigated and analyzed. Serum samples were collected from patients after receiving informed consent. Exosomes were extracted from the serum, and electron microscopy was used to observe the ultrastructure of exosomes. Quantitative PCR was used to detect lncRNA HEIH gene expression in serum and exosomes.
RESULTS
The changes in the ALT, GGT, HDL, INR, Alb and AFP levels in the patients with HCV-induced cirrhosis and HCV-related HCC were statistically significant. In patients with HCV-related HCC, lncRNA-HEIH expression in serum and exosomes was increased, but the ratio of lncRNA-HEIH expression in serum versus exosomes was decreased compared to patients with CHC.
背景
慢性丙型肝炎（CHC）是一种由丙型肝炎病毒（HCV）感染引起的传染性肝脏疾病。CHC 最严重的后果是 HCV 相关的肝细胞癌（HCC）。
目的
阐明血清和外泌体中长链非编码 RNA HEIH 表达在 HCV 相关 HCC 发展中的临床意义。
方法
本研究纳入了 2016 年 1 月至 2016 年 9 月在湖州市中心医院就诊的 35 例 CHC、22 例 HCV 诱导的肝硬化和 10 例 HCV 相关 HCC 患者。调查和分析了基本患者信息、临床血清学指标和临床影像学数据。患者在知情同意后采集血清样本。从血清中提取外泌体，用电子显微镜观察外泌体的超微结构。用定量 PCR 检测血清和外泌体中 lncRNA HEIH 基因的表达。
结果
HCV 诱导的肝硬化和 HCV 相关 HCC 患者的 ALT、GGT、HDL、INR、Alb 和 AFP 水平变化有统计学意义。在 HCV 相关 HCC 患者中，血清和外泌体中的 lncRNA-HEIH 表达增加，但与 CHC 患者相比，血清中 lncRNA-HEIH 表达与外泌体的比值降低。
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