Milk Science Research Institute, Megmilk Snow Brand Co. Ltd, 1-1-2 Minamidai, Kawagoe, Japan.
Milk Science Research Institute, Megmilk Snow Brand Co. Ltd, 1-1-2 Minamidai, Kawagoe, Japan.
Food Chem. 2018 Apr 15;245:1218-1223. doi: 10.1016/j.foodchem.2017.11.009. Epub 2017 Nov 3.
Firstly, we report the localization analysis of the lipid components of a water-in-oil (W/O) semi-solid emulsion by mass spectrometry imaging (MSI). Uniform emulsion droplets were prepared using microchannel emulsification devices with lecithin, stearic acid-binding monoglyceride (St-MAG), and polyglycerol polyricinoleate (PGPR) as emulsifiers. The mass image gives us the localization of phosphatidylcholine (PC) in lecithin, St-MAG, tripalmitin (PPP), medium-chain triglyceride (MCT), and high-melting-point triglyceride tristearin (C18-TAG). PC, St-MAG, and PPP were localized at the interface with the dispersed water droplets. PC and PPP took the same localized position, suggesting an interaction between PC and PPP at the interface. Conversely, PC existed in other regions with St-MAG. MSI revealed multiple target molecules in fat products in a single measurement, and it is expected to reveal fat crystallization at the emulsion interfaces, which will clarify the mechanisms related to the physical properties of high-fat products such as fat spread and butter.
首先，我们通过质谱成像（MSI）报告了油包水（W/O）半固体乳液中脂质成分的定位分析。使用微通道乳化设备，以卵磷脂、硬脂酸结合单甘油酯（St-MAG）和聚甘油蓖麻醇酸酯（PGPR）作为乳化剂，制备均匀的乳液液滴。质量图像给出了卵磷脂、St-MAG、三棕榈酸甘油酯（PPP）、中链甘油三酯（MCT）和高熔点甘油三酯三硬脂酸酯（C18-TAG）中磷脂酰胆碱（PC）的定位。PC、St-MAG 和 PPP 定位在与分散的水相接触的界面处。PC 和 PPP 定位在相同的位置，这表明 PC 和 PPP 之间在界面处存在相互作用。相反，PC 存在于与 St-MAG 不同的其他区域。MSI 在单次测量中揭示了脂肪产品中的多个目标分子，预计可以揭示乳液界面处的脂肪结晶，这将阐明与高脂肪产品（如涂抹脂和黄油）的物理性质相关的机制。
