Faculty of Food and Agricultural Sciences, Fukushima University, Fukushima, Japan.
Department of Bioscience and Biotechnology, Fukui Prefectural University, Eiheiji, Japan.
Methods Mol Biol. 2022;2437:127-139. doi: 10.1007/978-1-0716-2030-4_9.
Plant hormones can act in synergistic and antagonistic ways in response to biotic and abiotic stresses and during plant growth and development. Thus, a technique is needed to simultaneously determine the distribution and concentration of several plant hormones. A relatively new technology, mass spectrometry imaging (MSI), enables the direct mapping and imaging of biomolecules on tissue sections. MSI permits simultaneous detection of multiple analytes on a single section of plant tissue, even in the absence of target-specific markers such as antibodies. Recently, MSI has been used to localize multiple, small molecule (m/z < 500) plant hormones by the nanoparticle-assisted laser desorption/ionization (Nano-PALDI) mass spectrometry (MS) method. Here, we illustrate a technology for multiple-hormone imaging using Nano-PALDI MSI and discuss its potential in investigating the role of hormone signaling in plant development and stress responses.
植物激素可以在生物和非生物胁迫以及植物生长发育过程中以协同和拮抗的方式发挥作用。因此，需要一种技术来同时测定几种植物激素的分布和浓度。一种相对较新的技术，质谱成像（MSI），可以直接在组织切片上对生物分子进行定位和成像。MSI 允许在植物组织的单个切片上同时检测多种分析物，即使在没有目标特异性标记物（如抗体）的情况下也是如此。最近，MSI 已被用于通过纳米颗粒辅助激光解吸/电离（Nano-PALDI）质谱（MS）方法定位多种小分子（m/z<500）植物激素。在这里，我们展示了一种使用 Nano-PALDI MSI 进行多激素成像的技术，并讨论了其在研究激素信号在植物发育和应激反应中的作用的潜力。
