School of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China.
Food Chem. 2018 Apr 15;245:500-507. doi: 10.1016/j.foodchem.2017.10.126. Epub 2017 Nov 5.
Soy protein hydrolyzate has been used as supplements in wheat flour to enhance the nutritional value of its products, but it may negatively affect the gluten properties simultaneously. In order to explore the mechanism of this effect, protein characteristics including disulfide bond, protein composition, intermolecular force of dough proteins, and atomic force microscope images of gluten were obtained. Results showed that disulfide bonds in dough increased when soy protein hydrolyzate was added, but glutenin macropolymer decreased. Atomic force microscope images showed that gluten were weakened by soy protein hydrolyzate. Based on these results, a model was developed to describe the interaction between soy protein hydrolyzates and wheat proteins: soy protein hydrolyzates linked with wheat proteins through disulfide bond, disrupted the glutenins polymerization, thus hindered gluten networks formation. The interaction between wheat proteins and soy protein hydrolyzates in noodle making dough could be described with this model reasonably.
大豆蛋白水解物已被用作面粉的补充物,以提高其产品的营养价值,但它可能同时对面筋特性产生负面影响。为了探究这种影响的机制,我们获得了包括二硫键、蛋白质组成、面团蛋白质分子间作用力和面筋原子力显微镜图像在内的蛋白质特性。结果表明,添加大豆蛋白水解物会增加面团中的二硫键,但麦谷蛋白大聚合物减少。原子力显微镜图像显示,面筋被大豆蛋白水解物削弱。基于这些结果,我们建立了一个模型来描述大豆蛋白水解物和小麦蛋白之间的相互作用:大豆蛋白水解物通过二硫键与小麦蛋白结合,破坏了麦谷蛋白的聚合,从而阻碍了面筋网络的形成。该模型可以合理地描述面条制作面团中小麦蛋白和大豆蛋白水解物之间的相互作用。