College of Life Sciences, China Jiliang University, 258 Xueyuan Street, Xiasha High Education Area, Hangzhou 310018, PR China.
College of Life Sciences, China Jiliang University, 258 Xueyuan Street, Xiasha High Education Area, Hangzhou 310018, PR China.
Food Chem. 2018 Apr 15;245:641-645. doi: 10.1016/j.foodchem.2017.08.107. Epub 2017 Sep 1.
A simple, sensitive, accurate and affordable rapid detection of meat species authentication is urgently needed in food industry. In this study, a cross priming amplification (CPA) combining nucleic acid test strip (CPA-Strip) assay for rapid detection of mutton from meat mixture were developed and its feasibility was investigated. In an isothermal CPA system, cytochrome b (cytb) gene as target was amplified at 63°C for 60min. The nucleic acid strip was able to show the corresponding test line in the presence of target gens in 5min. Non-targeting gene interference was not evident. The CPA-Strip has been applied for the detection of 0.1-100% mutton in a thermal treated meat mixtures with a detection limit of a detect limit of 1%. CPA-Strip assay would be a promising simple, rapid and sensitive method for identification of target species in raw and processed meat mixtures.
在食品行业，急需一种简单、灵敏、准确且经济实惠的肉类物种鉴定快速检测方法。本研究开发了一种结合核酸试纸条（CPA-Strip）的交叉引物扩增（CPA）检测方法，用于快速检测肉混合物中的羊肉，并对其可行性进行了研究。在等温的 CPA 系统中，以细胞色素 b（cytb）基因为靶标，在 63°C 下扩增 60min。在 5min 内，若存在目标基因，核酸试纸条上就会出现相应的检测线。非靶向基因干扰并不明显。CPA-Strip 已应用于热加工肉混合物中 0.1-100%羊肉的检测，检测限为 1%。CPA-Strip 检测法有望成为一种简单、快速、灵敏的方法，可用于鉴定生肉和加工肉混合物中的目标物种。
