Department of Biosphere Sciences, Kobe College, 4-1 Okadayama, Nishinomiya, Hyogo 662-8505, Japan.
Department of Biosphere Sciences, Kobe College, 4-1 Okadayama, Nishinomiya, Hyogo 662-8505, Japan.
Food Chem. 2018 Apr 15;245:750-755. doi: 10.1016/j.foodchem.2017.11.119. Epub 2017 Dec 5.
Amino acids exert characteristic antioxidant activities depending on the properties of their side residues. The hydrophobic residues were effective against peroxyl radical, while acidic residues and their analogs were effective against peroxynitrite. Peptides containing tyrosine showed different activities against different reactive oxygen species (ROS) and/or reactive nitrogen species (RNS). The number and position of tyrosine did not affect the antioxidant activity against hypochlorite ion. Against the peroxyl radical, the number of tyrosine residues affected the antioxidant activity, while its position did not have a significant effect. The tyrosine position was an important factor for the antioxidant activity against peroxynitrite. The peptide GWWW showed higher antioxidant activity against peroxyl radical than tryptophan at concentrations below 25 µM, and high activity against peroxynitrite at 250 µM. Our results suggest that antioxidant peptides against a specific target ROS or RNS can be designed based on the characteristics of the amino acid side chains.
氨基酸根据其侧链残基的性质发挥出特有的抗氧化活性。疏水性残基对过氧自由基有效，而酸性残基及其类似物对过氧亚硝酸盐有效。含酪氨酸的肽对不同的活性氧（ROS）和/或活性氮（RNS）显示出不同的活性。酪氨酸的数量和位置并不影响其对次氯酸根离子的抗氧化活性。在过氧自由基方面，酪氨酸残基的数量影响抗氧化活性，而其位置没有显著影响。酪氨酸的位置是其对过氧亚硝酸盐的抗氧化活性的一个重要因素。在低于 25µM 的浓度下，肽 GWWW 对过氧自由基的抗氧化活性高于色氨酸，在 250µM 时对过氧亚硝酸盐具有高活性。我们的结果表明，针对特定目标 ROS 或 RNS 的抗氧化肽可以根据氨基酸侧链的特性来设计。
