Department of Biosphere Sciences, School of Human Sciences, Kobe College, 4-1 Okadayama, Nishinomiya City, Hyogo 662-8505, Japan.
Plant Cell Rep. 2013 Mar;32(3):349-57. doi: 10.1007/s00299-012-1368-z. Epub 2012 Nov 16.
Antioxidant activity of seven leafy vegetables and four beans against five reactive oxygen species and reactive nitrogen species was clearly characterized with a protocol using myoglobin as a reporter probe. Antioxidant activity of seven leafy vegetables and four beans against peroxyl radical, hydroxyl radical, hypochlorite ion, and peroxynitrite ion has been measured using myoglobin as a reporter probe (myoglobin method). Conventional DPPH method was also used to evaluate antioxidant activity of the samples. Difference of activity against different reactive oxygen species (ROS) and reactive nitrogen species (RNS) was characterized by plotting the data in a 5-axe cobweb chart. This plot clearly showed the characteristics of the antioxidant activity of the leafy vegetables and the beans. The samples examined in this work were categorized into four groups. (1) The samples showed high antioxidant activity against all ROS and RNS: daikon sprout, spinach, Qing-geng-cai, and onion. (2) The samples showed high antioxidant activity against peroxyl radical: red bean and soy bean. (3) The samples showed high antioxidant against hypochlorite ion: broccoli floret, cabbage, and Chinese cabbage. (4) The samples showed weak antioxidant activity against all ROS and RNS: cowpea and common beans. Our protocol is probably useful to characterize antioxidant activity of the crops of different cultivars, the crops obtained in different growing environments and growing seasons, the crops harvested at different age, and the crops stored in the different conditions, as well as the changes of activity during cooking process of the crops.
使用肌红蛋白作为报告探针，明确描述了七种叶菜和四种豆类对五种活性氧和活性氮物质的抗氧化活性。使用肌红蛋白作为报告探针（肌红蛋白法）测量了七种叶菜和四种豆类对过氧自由基、羟自由基、次氯酸根离子和过氧亚硝酸盐离子的抗氧化活性。还使用传统的 DPPH 法评估了样品的抗氧化活性。通过在 5 轴蛛网图中绘制数据，可表征对不同活性氧（ROS）和活性氮（RNS）的活性差异。该图清楚地显示了叶菜和豆类的抗氧化活性特征。在这项工作中检查的样品分为四组。（1） 这些样品对所有 ROS 和 RNS 均表现出高抗氧化活性：萝卜芽、菠菜、青梗菜和洋葱。（2） 这些样品对过氧自由基表现出高抗氧化活性：红豆和大豆。（3） 这些样品对次氯酸根离子表现出高抗氧化活性：花椰菜、白菜和结球甘蓝。（4） 这些样品对所有 ROS 和 RNS 表现出较弱的抗氧化活性：豇豆和普通豆。我们的方案可能有助于表征不同品种作物、不同生长环境和生长季节收获的作物、不同收获年龄的作物以及不同条件下储存的作物的抗氧化活性，以及在烹饪过程中活性的变化。
