Treviño-Rangel Rogelio de J, Peña-López Cynthia D, Hernández-Rodríguez Pedro A, Beltrán-Santiago Dinael, González Gloria M
Department of Microbiology, School of Medicine, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Infectology Service, Department of Internal Medicine, University Hospital "Dr. José E. González", Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Rev Iberoam Micol. 2018 Jan-Mar;35(1):11-16. doi: 10.1016/j.riam.2017.01.005. Epub 2017 Dec 26.
Candidemia is one of the most common nosocomial infections globally and it is associated with considerable excess mortality and costs. Abreast, biofilm-forming strains are associated with even higher mortality rates and poor prognosis for the patient.
To evaluate a possible association between the biofilm-forming capability of Candida bloodstream isolates and the clinical evolution in patients with candidemia.
An observational, retrospective study was conducted at a tertiary care university hospital during 9 years (2006-2015). The biofilm quantitation of the Candida bloodstream isolates was determined by crystal violet staining and XTT reduction assay.
A total of 218 cases of candidemia had been diagnosed and 89 isolates were obtained. The mortality rate was 36% and the main risk factors were antibiotic exposure and the use of catheters. Candida tropicalis (52.8%) was the most frequent species, followed by Candida albicans (30.4%), Candida parapsilosis sensu stricto (10.1%), Candida orthopsilosis (3.4%), Candida krusei (2.2%) and Candida glabrata sensu stricto (1.1%). All the strains were biofilm producers, which is an important contribution to the patient's mortality. C. tropicalis showed the highest production of biomass biofilm, whereas C. glabrata exhibited the highest metabolic activity.
This study contributes to expand the knowledge about the local epidemiology of candidemia and highlights the impact of Candida biofilm on patient's outcome.
念珠菌血症是全球最常见的医院感染之一，与相当高的额外死亡率和成本相关。此外，形成生物膜的菌株与更高的死亡率和患者的不良预后有关。
评估念珠菌血流分离株的生物膜形成能力与念珠菌血症患者临床转归之间的可能关联。
在一家三级护理大学医院进行了一项为期9年（2006 - 2015年）的观察性回顾性研究。通过结晶紫染色和XTT还原试验测定念珠菌血流分离株的生物膜定量。
共诊断出218例念珠菌血症病例，获得89株分离株。死亡率为36%，主要危险因素是抗生素暴露和导管使用。热带念珠菌（52.8%）是最常见的菌种，其次是白色念珠菌（30.4%）、近平滑念珠菌（10.1%）、正平滑念珠菌（3.4%）、克柔念珠菌（2.2%）和光滑念珠菌（1.1%）。所有菌株均为生物膜产生菌，这对患者的死亡率有重要影响。热带念珠菌生物膜的生物量产生最高，而光滑念珠菌表现出最高的代谢活性。
本研究有助于扩大对念珠菌血症局部流行病学的认识，并突出念珠菌生物膜对患者预后的影响。
