School of Chemical Engineering and Environment, North University of China, Taiyuan 030051, PR China; Department of Environment and Safety Engineering, Taiyuan Institute of Technology, Taiyuan 030008, PR China; Everwise Biotechnology Co. Ltd., Taiyuan, 030008, PR China.
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, PR China.
Vaccine. 2018 Jan 29;36(5):723-728. doi: 10.1016/j.vaccine.2017.12.026. Epub 2017 Dec 26.
As one of the most challenging problems in swine industry, piglet diarrhea has caused huge economic loss globally. Currently, vaccination is the most effective way of controlling enterotoxigenic Escherichia coli (ETEC) diarrhea. However, existing commercial vaccines could not provide broad protection against different types of ETEC. In this study, we mixed a enterotoxin fusion protein SLS (STa-LTB-STb) with the main fimbrial F4ac and F5 antigens as a novel multivalent vaccine candidate. Then an overall evaluation of this vaccine candidate against ETEC was carried out in a pig model. We found that the IgG titers in serum as well as colostrum in all the vaccinated sows were significantly higher than that in the control group (P < 0.05). By using a sensory evaluation method, we demonstrated that piglets in the vaccinated group exhibited significantly healthier status than the unimmunized group. Moreover, in response to F41 + ETEC challenge, none of the piglets with the vaccine candidate experienced diarrhea, whereas 30% of the piglets suffered without vaccination. In conclusion, these results showed that the candidate vaccine could elicit multiple high-titer antibodies against all the main virulence factors and provide a broad and effective protection against ETEC diarrhea.
作为养猪业面临的最具挑战性的问题之一，仔猪腹泻在全球范围内造成了巨大的经济损失。目前，疫苗接种是控制肠毒素性大肠杆菌（ETEC）腹泻最有效的方法。然而，现有的商业疫苗不能为不同类型的 ETEC 提供广泛的保护。在本研究中，我们将肠毒素融合蛋白 SLS（STa-LTB-STb）与主要菌毛 F4ac 和 F5 抗原混合，作为一种新型多价疫苗候选物。然后，我们在猪模型中对该疫苗候选物针对 ETEC 的效果进行了全面评估。我们发现，所有接种母猪的血清和初乳中的 IgG 滴度均明显高于对照组（P<0.05）。通过使用感官评估方法，我们证明接种组的仔猪比未免疫组的仔猪表现出明显更健康的状态。此外，在 F41+ETEC 攻毒后，接种候选疫苗的仔猪无一出现腹泻，而未接种疫苗的仔猪中有 30%出现腹泻。总之，这些结果表明，该候选疫苗可以针对所有主要毒力因子产生多种高滴度抗体，并为 ETEC 腹泻提供广泛有效的保护。
