Zhang Yanyan, Tian Xiaorong, Dong Yi, Li Rui, Shen Meng, Yi Dan, Wu Tao, Wang Lei, Zhao Di, Hou Yongqing
Hubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Wuhan Polytechnic University, Wuhan, China.
Front Cell Infect Microbiol. 2023 Nov 14;13:1284166. doi: 10.3389/fcimb.2023.1284166. eCollection 2023.
Enterotoxigenic (ETEC), an important intestinal pathogen, poses a significant threat to the intestinal health of piglets. (BC), a potential feed additive, can improve the intestinal function of piglets. However, the effects of BC on growth performance and intestinal function in ETEC-infected piglets are still unclear. In this study, 24 7-day-old piglets were randomly assigned to three treatment groups: control group (fed a basal diet), ETEC group (fed a basal diet and challenged with ETEC K88) and BC+ETEC group (fed a basal diet, orally administered BC, challenged with ETEC K88). During Days 1-6 of the trial, piglets in the BC+ETEC group were orally administered BC (1×10CFU/kg). On Day 5 of the trial, piglets in the ETEC and BC+ETEC groups were orally administered ETEC K88 (5×10CFU/piglet). Blood, intestinal tissue, and content samples were collected from the piglets on Day 7 of the trial.
The average daily feed intake in the ETEC group was significantly reduced compared to that of the control group. Further research revealed that ETEC infection significantly damaged the structure of the small intestine. Compared to the control group, the villus height and surface area of the jejunum, the ratio of villus height to crypt depth in the duodenum and jejunum, and the activities of catalase and total superoxide dismutase in the jejunum were significantly reduced. Additionally, the levels of myeloperoxidase in the jejunum, malondialdehyde in the plasma and jejunum, and intestinal epithelial apoptosis were significantly increased in the ETEC group. However, BC supplementation had significantly mitigated these negative effects in the BC+ETEC group by Day 7 of the trial. Moreover, BC supplementation improved the gut microbiota imbalance by reversing the decreased numbers of , and in jejunum and , and in the colon, as well as the increased number of in the jejunum induced by ETEC K88.
Overall, BC supplementation reduced the decline in average daily feed intake in ETEC K88-infected piglets by attenuating intestinal epithelial apoptosis and oxidative stress and regulating the gut microbiota. This suggests that BC may be used to prevent intestinal infections caused by ETEC in piglets.
产肠毒素大肠杆菌（ETEC）是一种重要的肠道病原体，对仔猪的肠道健康构成重大威胁。丁酸梭菌（BC）作为一种潜在的饲料添加剂，可改善仔猪的肠道功能。然而，BC对ETEC感染仔猪生长性能和肠道功能的影响仍不清楚。在本研究中，将24头7日龄仔猪随机分为三个处理组：对照组（饲喂基础日粮）、ETEC组（饲喂基础日粮并接种ETEC K88）和BC + ETEC组（饲喂基础日粮，口服BC，接种ETEC K88）。在试验的第1至6天，BC + ETEC组的仔猪口服BC（1×10CFU/kg）。在试验的第5天，ETEC组和BC + ETEC组的仔猪口服ETEC K88（5×10CFU/仔猪）。在试验的第7天从仔猪采集血液、肠道组织和内容物样本。
与对照组相比，ETEC组的平均日采食量显著降低。进一步研究表明，ETEC感染显著破坏了小肠结构。与对照组相比，空肠的绒毛高度和表面积、十二指肠和空肠的绒毛高度与隐窝深度之比以及空肠中的过氧化氢酶和总超氧化物歧化酶活性显著降低。此外，ETEC组空肠中的髓过氧化物酶水平、血浆和空肠中的丙二醛水平以及肠道上皮细胞凋亡显著增加。然而，到试验第7天，补充BC在BC + ETEC组中显著减轻了这些负面影响。此外，补充BC通过逆转ETEC K88诱导的空肠中、和数量的减少以及结肠中、和数量的减少以及空肠中数量的增加，改善了肠道微生物群失衡。
总体而言，补充BC通过减轻肠道上皮细胞凋亡和氧化应激以及调节肠道微生物群，减少了ETEC K88感染仔猪平均日采食量的下降。这表明BC可用于预防仔猪由ETEC引起的肠道感染。
