Department of Marine Biology and Environmental Science, University of Miyazaki, Miyazaki, Japan; Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Fish Shellfish Immunol. 2018 Mar;74:26-34. doi: 10.1016/j.fsi.2017.12.036. Epub 2017 Dec 26.
Supplementation of prebiotic carbohydrates can act as a potent immunomodulator and have the efficacy to induce immune-related genes which are involved in host defense. Pure β-1,4-mannobiose (MNB) showed activation of prophenoloxidase system of shrimp hemocytes in vitro. The resistance of kuruma shrimp Marsupenaeus japonicus against Vibrio parahaemolyticus was examined after the shrimp were fed with 0 (control), 0.02, 0.2, and 2% MNB supplemented diets. The results showed significantly higher survival rates in MNB supplemented shrimp than those of the control one from 2 to 12 days post challenge. In another experiment, the hemocyte count, ROS production, phagocytic, phenoloxidase and bactericidal activities, and expression of immune-related genes were investigated in the control and MNB supplemented groups at day 1, 4, 6, 8 and 11 of the feeding. These immune parameters were significantly enhanced in MNB supplemented groups. Furthermore, the gene expression analysis showed that transcripts of lysozyme, crustin, penaeidin and TNF were significantly up-regulated in hemolymph, lymphoid organs and intestines of MNB treated shrimp. Overall, the results provided evidence that MNB supplementation could improve the immune response and increase shrimp resistance against V. parahaemolyticus infection.
添加益生元碳水化合物可以作为一种有效的免疫调节剂，诱导参与宿主防御的免疫相关基因。纯β-1,4-甘露二糖（MNB）在体外能激活虾血细胞的酚氧化酶原系统。在给虾投喂 0（对照）、0.02、0.2 和 2%MNB 补充饲料后，检测了日本对虾（Marsupenaeus japonicus）对副溶血弧菌的抗性。结果显示，在攻毒后 2 至 12 天，投喂 MNB 的虾的存活率明显高于对照组。在另一个实验中，在投喂的第 1、4、6、8 和 11 天，检测了对照组和 MNB 补充组的血细胞计数、ROS 产生、吞噬作用、酚氧化酶和杀菌活性以及免疫相关基因的表达。MNB 补充组的这些免疫参数显著增强。此外，基因表达分析显示，MNB 处理虾的血淋巴、淋巴器官和肠道中溶菌酶、甲壳质、 penaeidin 和 TNF 的转录物显著上调。总的来说，这些结果提供了证据表明，MNB 补充可以改善免疫反应并提高虾对副溶血弧菌感染的抵抗力。
