Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University (Bogor Agricultural University), Bogor, 16680, Indonesia; Department of Aquaculture, Faculty of Fisheries and Marine Science, Halu Oleo University, Kendari, 93232, Indonesia.
Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University (Bogor Agricultural University), Bogor, 16680, Indonesia.
Fish Shellfish Immunol. 2020 Jul;102:218-227. doi: 10.1016/j.fsi.2020.03.066. Epub 2020 Apr 5.
This study investigated the influence of Eleutherine bulbosa (Mill.) Urb. on the immune responses, bacterial population in the intestines, and resistance of white shrimp, Litopenaeus vannamei, against infection with Vibrio parahaemolyticus. Shrimp were fed with three dosages of powder, at 6.25 g kg-1 (P6.25), 12.5 g kg-1 (P12.5), and 25 g kg-1 (P25). One dosage of the crude extract was provided, 1.25 g kg-1 (E1.25), and the controls without administration of E. bulbosa consisted of a positive control (PC) and a negative control (NC). Feed supplementation was carried out for 30 days; then shrimp from all treatments were challenged by intramuscular injection with V. parahaemolyticus (10 cfu/mL), except for the NC. The results showed that supplementation with the powder and extract of E. bulbosa for 30 days resulted in significantly higher (P < 0.05) immune responses (total hemocyte count (THC), phenoloxidase activity (PO), respiratory bursts (RBs)), gene expression (prophenoloxidase (proPO), lipopolysaccharide- and β-1,3-glucan-binding protein (LGBP)), and total bacterial count (TBC) compared to PC/NC. In post challenge testing, there were significantly higher levels for THC, PO, RBs, proPO, LGBP, and PE (peroxinetin), and the treatments were able to suppress V. parahaemolyticus in the intestines, hepatopancreas, and muscles and to reduce damage to the muscles and hepatopancreas. The survival rate with P12.5 was significantly higher compared to the other treatments. It was concluded that the shrimp receiving supplementation with the powder and extract of E. bulbosa had increased immunity and resistance against V. parahaemolyticus infection, with the best dosage being the P12.5 treatment.
本研究探讨了 Eleutherine bulbosa (Mill.) Urb. 对免疫反应、肠道细菌种群以及南美白对虾 (Litopenaeus vannamei) 对副溶血弧菌感染的抵抗力的影响。虾用三种剂量的粉末喂养，分别为 6.25g/kg（P6.25）、12.5g/kg（P12.5）和 25g/kg（P25）。还提供了一个粗提取物剂量，即 1.25g/kg（E1.25），未给予 Eleutherine bulbosa 的对照组包括阳性对照组（PC）和阴性对照组（NC）。饲料补充持续 30 天；然后，除 NC 组外，所有处理组的虾都通过肌肉注射副溶血弧菌（10cfu/mL）进行攻毒。结果表明，补充 Eleutherine bulbosa 粉末和提取物 30 天后，与 PC/NC 相比，免疫反应（总血细胞计数（THC）、酚氧化酶活性（PO）、呼吸爆发（RB））、基因表达（原酚氧化酶（proPO）、脂多糖和β-1,3-葡聚糖结合蛋白（LGBP））和总细菌计数（TBC）显著升高（P<0.05）。在攻毒后测试中，THC、PO、RB、proPO、LGBP 和 PE（过氧化物酶）的水平显著升高，并且处理能够抑制肠道、肝胰腺和肌肉中的副溶血弧菌，并减少肌肉和肝胰腺的损伤。与其他处理相比，P12.5 处理的存活率显著更高。结论是，接受 Eleutherine bulbosa 粉末和提取物补充的虾的免疫力和对副溶血弧菌感染的抵抗力增强，最佳剂量为 P12.5 处理。
