Post-Graduation Program in Health Science, Federal University of Ceará, Sobral, CE, Brazil.
Post-Graduation Program RENORBIO, Federal University of Ceará, Sobral, CE, Brazil.
Clin Oral Investig. 2018 Jul;22(6):2175-2185. doi: 10.1007/s00784-017-2308-7. Epub 2017 Dec 29.
The aim of this work was to evaluate the anti-inflammatory and antiresorptive effects of Calendula officinalis (CLO) on alveolar bone loss (ABL) in rats.
Male Wistar rats were subjected to ABL by ligature with nylon thread around the second upper left molar. The contralateral hemimaxillae were used as control. Rats received saline solution (SAL) or CLO (10, 30, or 90 mg/kg) 30 min before ligature and daily until the 11th day. The maxillae were removed and prepared for macroscopic, radiographic, micro-tomographic, histopathologic, histometric analysis, and immunohistochemical localization of receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG). The gingival tissues were used to quantify the myeloperoxidase (MPO) activity, tumor necrosis factor-alpha (TNF-α), and interleukin-1β (IL-1β) concentrations by ELISA. Blood samples were collected for leukogram and to evaluate the bone-specific alkaline phosphatase (BALP) activity and serum levels of aspartate and alanine transaminases (AST/ALT).
The bone loss induced by 11 days of ligature induced bone loss, reduced levels of BALP, leukocyte infiltration, increased MPO activity, gingival concentrations of TNF-α and IL-1β, and RANKL while reduced OPG immunoexpressions in the periodontal tissue and leukocytosis. Of the CLO, 90 mg/kg reduced bone loss, neutrophilia, the levels of pro-inflammatory mediators, and RANKL expression, while it increased OPG immunopositive cells and BALP serum levels, when compared to SAL. CLO did not affect either kidney or liver function, indicated by serum AST/ALT levels.
The present data suggests that CLO reduced inflammatory bone resorption in experimental periodontitis, which may be mediated by its anti-inflammatory properties and its effects on bone metabolism.
CLO can be a potential therapeutical adjuvant in the treatment of periodontitis.
本研究旨在评估金盏花（CLO）对大鼠牙槽骨丧失（ABL）的抗炎和抗吸收作用。
雄性 Wistar 大鼠通过结扎第二左上磨牙周围的尼龙线导致 ABL。对侧上颌骨用作对照。大鼠在结扎前 30 分钟给予生理盐水（SAL）或 CLO（10、30 或 90mg/kg），并在第 11 天前每天给予。上颌骨被取出并准备进行宏观、放射照相、微断层照相、组织病理学、组织计量分析以及核因子 kappa-B 配体（RANKL）和骨保护素（OPG）的免疫组织化学定位。用 ELISA 定量测定牙龈组织中髓过氧化物酶（MPO）活性、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的浓度。收集血液样本用于白细胞计数，并评估骨特异性碱性磷酸酶（BALP）活性以及血清天门冬氨酸和丙氨酸转氨酶（AST/ALT）水平。
结扎 11 天引起的骨丢失导致 BALP 水平降低、白细胞浸润增加、MPO 活性增加、牙龈 TNF-α 和 IL-1β 浓度增加以及牙周组织中 RANKL 减少和白细胞增多。CLO（90mg/kg）降低了骨丢失、中性粒细胞增多、促炎介质水平和 RANKL 表达，同时增加了 OPG 免疫阳性细胞和 BALP 血清水平，与 SAL 相比。CLO 对肾脏或肝脏功能没有影响，血清 AST/ALT 水平表明。
本研究数据表明，CLO 减少了实验性牙周炎中的炎症性骨吸收，这可能与其抗炎特性及其对骨代谢的影响有关。
CLO 可能是治疗牙周炎的潜在治疗性辅助药物。
