Ochoa-Díaz Margarita M, Daza-Giovannetty Silvana, Gómez-Camargo Doris
Faculty of Medicine, Tropical Medicine Doctorate, UNIMOL, University of Cartagena, Cartagena, Colombia.
Methods Mol Biol. 2018;1734:13-20. doi: 10.1007/978-1-4939-7604-1_2.
Bacterial genotyping methods have been used in several areas of microbiology and have facilitated the identification of bacterial strains, as well as the study of virulence and resistance factors, epidemiological surveillance, among others. Constituting, in this way, as complementary or alternative to phenotypic methods. This chapter reviews some of the methods of bacterial genotyping used nowadays, explains briefly how they are performed, and highlights some limitations and advantages they have.
细菌基因分型方法已应用于微生物学的多个领域,有助于细菌菌株的鉴定,以及毒力和耐药因子的研究、流行病学监测等。从而构成了表型方法的补充或替代方法。本章回顾了当今使用的一些细菌基因分型方法,简要解释了它们的操作方式,并强调了它们的一些局限性和优点。