Amador Cristina Isabel, Sternberg Claus, Jelsbak Lars
Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark.
Methods Mol Biol. 2018;1734:131-158. doi: 10.1007/978-1-4939-7604-1_12.
Complex interactions between pathogenic bacteria, the microbiota, and the host can modify pathogen physiology and behavior. We describe two different experimental approaches to study microbe-microbe interactions in in vitro systems containing surface-associated microbial populations. One method is the application of RNA sequencing (RNA-seq) to determine the transcriptional changes in pathogenic bacteria in response to microbial interspecies interactions. The other method combines flow cell devices for bacterial cultivation and growth with high-resolution bioimaging to analyze the microscale structural organization of interacting microbial populations within mixed-species biofilms.
病原菌、微生物群和宿主之间的复杂相互作用会改变病原体的生理和行为。我们描述了两种不同的实验方法，用于研究含有表面相关微生物群体的体外系统中的微生物-微生物相互作用。一种方法是应用RNA测序（RNA-seq）来确定病原菌响应微生物种间相互作用时的转录变化。另一种方法是将用于细菌培养和生长的流动细胞装置与高分辨率生物成像相结合，以分析混合物种生物膜中相互作用的微生物群体的微观结构组织。
