Mitra Arindam, Mukhopadhyay Suman
Department of Biological Sciences, School of Life Science and Biotechnology, Adamas University, Kolkata, India.
Division of Microbiology and Infectious Diseases HHS/NIH/National Institute of Allergy and Infectious Disease, Rockville, MD, 20852, USA.
Curr Res Pharmacol Drug Discov. 2022 Dec 29;4:100151. doi: 10.1016/j.crphar.2022.100151. eCollection 2023.
Biofilm refers to microbes that associate with each other or to a surface via self-synthesized exopolysaccharides and other surface-related structures. The presence of biofilms consisting of pathogenic microbes in the food and clinical environment can pose a threat to human health as microbes in biofilms are highly robust and are difficult to remove. Understanding the process of biofilm formation is crucial for the development of novel strategies to control or harness biofilm. The complex network of proteins, small RNA, and diverse molecules regulate biofilm formation at different steps in biofilm development, including triggering the switch from planktonic to sessile cells, maturation of biofilms, and eventual dispersion of microbes from the biofilms. Small non-coding RNAs are relatively small RNAs that are not translated into proteins and play diverse roles in metabolism, physiology, pathogenesis, and biofilm formation. In this review, we primarily focused on non-coding regulatory RNA that regulates biofilm formation in clinically relevant pathogens or threatens human health. Even though many ncRNA have recently been identified in Archaea, much characterization work remains. The mechanisms and regulatory processes controlled by ncRNA in prokaryotes are covered in this review.
生物膜是指通过自身合成的胞外多糖和其他与表面相关的结构相互关联或附着于表面的微生物。在食品和临床环境中，由致病微生物组成的生物膜的存在会对人类健康构成威胁，因为生物膜中的微生物具有很强的抗性且难以清除。了解生物膜的形成过程对于开发控制或利用生物膜的新策略至关重要。蛋白质、小RNA和各种分子组成的复杂网络在生物膜发育的不同阶段调节生物膜的形成，包括触发浮游细胞向固着细胞的转变、生物膜的成熟以及微生物最终从生物膜中扩散。小非编码RNA是相对较小的RNA，它们不被翻译成蛋白质，在代谢、生理学、发病机制和生物膜形成中发挥多种作用。在这篇综述中，我们主要关注调节临床相关病原体中生物膜形成或威胁人类健康的非编码调节RNA。尽管最近在古细菌中发现了许多非编码RNA，但仍有许多表征工作要做。本综述涵盖了原核生物中由非编码RNA控制的机制和调节过程。
