研究表面活性剂诱导的DNA分子的压缩-解压过程。
Studying compaction-decompaction of DNA molecules induced by surfactants.
作者信息
Li Xiaoyan, Sun Dan, Chen Yanyan, Wang Kaige, He Qingli, Wang Guiren
机构信息
State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, Laboratory of Optoelectronic Technology of Shaanxi Province, National Center for International Research of Photoelectric Technology & Nano-functional Materials and Application, Institute of Photonics and Photon-Technology, Northwest University, Xi'an 710069, China; Physics Department, Northwest University, Xi'an 710069, China.
State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, Laboratory of Optoelectronic Technology of Shaanxi Province, National Center for International Research of Photoelectric Technology & Nano-functional Materials and Application, Institute of Photonics and Photon-Technology, Northwest University, Xi'an 710069, China.
出版信息
Biochem Biophys Res Commun. 2018 Jan 22;495(4):2559-2565. doi: 10.1016/j.bbrc.2017.12.151. Epub 2017 Dec 27.
The mechanism and detailed processes of DNA compaction and decompaction are essential for the life activities, as well as for the researches in the molecular biology, genetics and biomedicine. The compaction of two kinds of DNA molecules caused by Cetyltrimethyl Ammonium Bromide (CTAB) and their decompaction induced with sodium dodecyl sulfate (SDS) or excessive amount of CTAB have been investigated with multiple perspectives such as the UV-VIS spectrophotometry, dynamic light scattering, and zeta potential. The compaction phenomenon of DNA can easily be observed when the CTAB combines with the DNA, not just when the molar ratio Q/Q is approximately equal to 1 as the conventional recognition, but also when Q/Q <1,DNA can be compacted; Molecular state of DNA is only changed in the conformational structure, but not in the chemical structure. Finally, a model is suggested to help catch on the biophysical mechanism of DNA chain conformational change.
DNA压缩和解压缩的机制及详细过程对于生命活动以及分子生物学、遗传学和生物医学研究至关重要。通过紫外可见分光光度法、动态光散射和zeta电位等多种视角，研究了十六烷基三甲基溴化铵（CTAB）引起的两种DNA分子的压缩以及十二烷基硫酸钠（SDS）或过量CTAB诱导的解压缩。当CTAB与DNA结合时，DNA的压缩现象很容易被观察到，不仅在传统认知中摩尔比Q/Q约等于1时，而且当Q/Q<1时，DNA也可以被压缩；DNA的分子状态仅在构象结构上发生变化，而不在化学结构上发生变化。最后，提出了一个模型以帮助理解DNA链构象变化的生物物理机制。
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