Zhu Changlai, Huang Jing, Xue Chengbin, Wang Yaxian, Wang Shengran, Bao Shuangxi, Chen Ruyue, Li Yuan, Gu Yun
Jiangsu Key Laboratory of Neuroregeneration, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Co-innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, JS, 226001, China.
Jiangsu Key Laboratory of Neuroregeneration, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Co-innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, JS, 226001, China.
Neurosci Res. 2018 Oct;135:21-31. doi: 10.1016/j.neures.2017.12.007. Epub 2017 Dec 27.
Extracellular/acellular matrix has been attracted much research interests for its unique biological characteristics, and ACM modified neural scaffolds shows the remarkable role of promoting peripheral nerve regeneration. In this study, skin-derived precursors pre-differentiated into Schwann cells (SKP-SCs) were used as parent cells to generate acellular(ACM) for constructing a ACM-modified neural scaffold. SKP-SCs were co-cultured with chitosan nerve guidance conduits (NGC) and silk fibroin filamentous fillers, followed by decellularization to stimulate ACM deposition. This NGC-based, SKP-SC-derived ACM-modified neural scaffold was used for bridging a 10 mm long rat sciatic nerve gap. Histological and functional evaluation after grafting demonstrated that regenerative outcomes achieved by this engineered neural scaffold were better than those achieved by a plain chitosan-silk fibroin scaffold, and suggested the benefits of SKP-SC-derived ACM for peripheral nerve repair.
细胞外/脱细胞基质因其独特的生物学特性而备受研究关注，且脱细胞基质修饰的神经支架在促进周围神经再生方面显示出显著作用。在本研究中，将预先分化为雪旺细胞的皮肤源性前体细胞（SKP-SCs）用作亲本细胞来生成脱细胞基质（ACM），以构建ACM修饰的神经支架。将SKP-SCs与壳聚糖神经引导导管（NGC）和丝素蛋白丝状填充物共培养，随后进行脱细胞处理以刺激ACM沉积。这种基于NGC的、源自SKP-SC的ACM修饰神经支架用于桥接10毫米长的大鼠坐骨神经缺损。移植后的组织学和功能评估表明，这种工程化神经支架实现的再生效果优于普通壳聚糖-丝素蛋白支架，并表明源自SKP-SC的ACM对周围神经修复有益。
