Li Qing, Yang Hongdan, He Lin, Wang Qun
College of Ecological Engineering, Guizhou University of Engineering Science, College Road, Bijie City, Guizhou Province 551700, China.
New York University Shanghai, No. 1555, Century Avenue, Shanghai 200120, China.
Gene. 2018 Mar 10;646:106-119. doi: 10.1016/j.gene.2017.12.044. Epub 2017 Dec 27.
Spermatogenesis involves a series of process including exiting from the mitotic cell cycle, entry into meiosis, completion of complex differentiation programs, and producing spermatozoa. Expression of various genes in an ordered manner, and interactions between various genes and their protein products, primarily controlled at the post-transcriptional level with DEAD-box RNA helicases playing a crucial role in germ cell development, are required for production of fertile sperm. Many members of this family have been deeply studied in spermatogenesis, such as DDX3X, DDX25 and DDX4, but few data are available on DDX52. In this study, we analyzed the expression patterns of Es-DDX52, Es-DDX6, Es-Vasa and Es-XRN1 both at mRNA and protein levels in different tissues and during gonadal development. It showed that Es-vasa, Es-DDX6 and Es-Xrn1, components of cytoplasmic foci P-bodies, have the similar transcriptional expression pattern, while Es-DDX52 has the reverse tendency. Furthermore, Es-DDX6 and Es-XRN1 proteins have the same localization in testicular tissues. Es-DDX52 mainly distributed in the cytoplasm of spermatogonia, only localized in the nucleus of early and middle spermatid and shifted to pre-acrosome vesicle (later developed into apical cap and acrosome tube) at both mRNA and protein levels. These results indicated that Es-DDX52 may participate in regulation of P-bodies and microtubules by Es-XRN1, and involved in the mitosis of spermatonia and spermatid differentiation.
精子发生涉及一系列过程，包括退出有丝分裂细胞周期、进入减数分裂、完成复杂的分化程序以及产生精子。为了产生可育精子，需要各种基因以有序的方式表达，以及各种基因与其蛋白质产物之间的相互作用，这些主要在转录后水平受到控制，其中DEAD-box RNA解旋酶在生殖细胞发育中起关键作用。该家族的许多成员已在精子发生过程中得到深入研究，如DDX3X、DDX25和DDX4，但关于DDX52的数据却很少。在本研究中，我们分析了Es-DDX52、Es-DDX6、Es-Vasa和Es-XRN1在不同组织以及性腺发育过程中mRNA和蛋白质水平的表达模式。结果表明，细胞质聚集体P小体的组成成分Es-vasa、Es-DDX6和Es-Xrn1具有相似的转录表达模式，而Es-DDX52则呈现相反的趋势。此外，Es-DDX6和Es-XRN1蛋白在睾丸组织中的定位相同。Es-DDX52主要分布在精原细胞的细胞质中，仅在早期和中期精子细胞的细胞核中定位，并且在mRNA和蛋白质水平上均转移至顶体前囊泡（后来发育为顶帽和顶体管）。这些结果表明，Es-DDX52可能通过Es-XRN1参与P小体和微管的调节，并参与精原细胞的有丝分裂和精子细胞的分化。
