Department of Thoracic Surgery, Affiliated Hospital of Nantong University, 20 Xisi Street, Nantong, 226001, China.
Sci Rep. 2023 Oct 13;13(1):17434. doi: 10.1038/s41598-023-44347-5.
Lung adenocarcinoma (LUAD) remains a leading cause of cancer-related morbidity and mortality globally. While DDX52, an ATP-dependent RNA helicase, plays a role in several biological processes, its specific involvement in LUAD is yet to be elucidated. We utilized ROC curves to determine DDX52's predictive potential for LUAD. Kaplan-Meier survival curves, along with univariate and multivariate Cox analyses, assessed the prognostic implications of DDX52 in LUAD. We constructed nomogram models to further delineate DDX52's influence on prognosis, employed GSEA for functional analysis, and used qRT-PCR to examine DDX52 expression in LUAD tissues. DDX52 expression was notably higher in LUAD tissues, suggesting its potential as a negative prognostic marker. We observed a direct relationship between DDX52 expression and advanced T and N stages, as well as higher grading and staging in LUAD patients. Cox analyses further underscored DDX52's role as an independent prognostic determinant for LUAD. GSEA insights indicated DDX52's influence on LUAD progression via multiple signaling pathways. Our nomogram, founded on DDX52 expression, effectively projected LUAD patient survival, as validated by calibration curves. Elevated DDX52 expression in LUAD tissues signals its potential as a poor prognostic marker. Our findings emphasize DDX52's role not only as an independent prognostic factor for LUAD but also as a significant influencer in its progression through diverse signaling pathways. The constructed nomogram also underscores the feasibility of predicting LUAD patient survival based on DDX52 expression.
肺腺癌 (LUAD) 仍然是全球癌症相关发病率和死亡率的主要原因。虽然 DDX52 是一种依赖 ATP 的 RNA 解旋酶，在多个生物学过程中发挥作用，但它在 LUAD 中的具体作用仍未阐明。我们利用 ROC 曲线来确定 DDX52 对 LUAD 的预测潜力。Kaplan-Meier 生存曲线以及单变量和多变量 Cox 分析评估了 DDX52 在 LUAD 中的预后意义。我们构建了列线图模型来进一步描绘 DDX52 对预后的影响，使用 GSEA 进行功能分析，并使用 qRT-PCR 检测 LUAD 组织中的 DDX52 表达。DDX52 在 LUAD 组织中的表达明显升高，表明其可能作为负预后标志物。我们观察到 DDX52 的表达与 LUAD 患者的晚期 T 和 N 分期以及更高的分级和分期之间存在直接关系。Cox 分析进一步强调了 DDX52 作为 LUAD 独立预后标志物的作用。GSEA 分析结果表明 DDX52 通过多种信号通路影响 LUAD 的进展。我们基于 DDX52 表达构建的列线图能够有效预测 LUAD 患者的生存，校准曲线验证了这一点。DDX52 在 LUAD 组织中的高表达表明其作为不良预后标志物的潜力。我们的研究结果强调了 DDX52 不仅作为 LUAD 的独立预后因素，而且作为通过多种信号通路影响其进展的重要因素的作用。构建的列线图还强调了基于 DDX52 表达预测 LUAD 患者生存的可行性。
