University of Warsaw, Faculty of Biology, Laboratory of Environmental Pollution Analysis, Miecznikowa 1, 02-096, Warsaw, Poland.
University of Warsaw, Faculty of Biology, Laboratory of Environmental Pollution Analysis, Miecznikowa 1, 02-096, Warsaw, Poland.
Chemosphere. 2018 Mar;195:722-726. doi: 10.1016/j.chemosphere.2017.12.123. Epub 2017 Dec 22.
The main aim of this study was the characterisation of novel Raoutella isolate, an iron-reducing and uranium-precipitating strain, originating from microbial mats occurring in the sediments of a closed down uranium mine in Kowary (SW Poland). Characterisation was done in the context of its potential role in the functioning of these mats and the possibility to use them in uranium removal/recovery processes. In our experiment, we observed the biological precipitation of iron and uranium's secondary minerals containing oxygen, potassium, sodium and phosphor, which were identified as ningyoite-like minerals. The isolated strain, Raoultella sp. SM1, was also able to dissimilatory reduce iron (III) and uranium (VI) in the presence of citrate as an electron donor. Our studies allowed us to characterise a new strain which may be used as a model microorganism in the study of Fe and U respiratory processes and which may be useful in the bioremediation of uranium-contaminated waters and sediments. During this process, uranium may be immobilised in ningyoite-like minerals and can then be recovered in nano/micro-particle form, which may be easily transformed to uraninite.
本研究的主要目的是对一种新型的 Raoutella 分离株进行特征描述，该分离株是一种铁还原和铀沉淀菌株，源自位于波兰西南部科瓦雷（Kowary）的一座关闭铀矿的沉积物中出现的微生物垫中。该特征描述是在其在这些垫子的功能中的潜在作用以及在铀去除/回收过程中使用它们的可能性的背景下进行的。在我们的实验中，我们观察到了铁和铀的次生含氧量、钾、钠和磷的生物沉淀，这些被鉴定为似镍矿矿物。分离株 Raoultella sp. SM1 也能够在以柠檬酸盐作为电子供体的情况下异化还原铁（III）和铀（VI）。我们的研究允许我们对一种新的菌株进行特征描述，该菌株可以用作铁和铀呼吸过程研究的模式微生物，并且在铀污染水和沉积物的生物修复中可能有用。在这个过程中，铀可以被固定在似镍矿矿物中，然后以纳米/微颗粒的形式回收，这可以很容易地转化为铀矿。
