Xu Shaozu, Luo Xuesong, Xing Yonghui, Liu Song, Huang Qiaoyun, Chen Wenli
1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
2Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China.
3 Biotech. 2019 Apr;9(4):120. doi: 10.1007/s13205-019-1649-0. Epub 2019 Mar 4.
A novel cadmium-resistant bacterium, sp. strain X13, recently isolated from heavy metal-contaminated soil, and this strain can synthesize CdS quantum dots using cadmium nitrate [Cd(NO)] and l-cysteine. Biomineralization of CdS by strain X13 can efficiently remove cadmium from aqueous solution. To illuminate the molecular mechanisms for the biosynthesis of CdS nanoparticle, the complete genome of sp. strain X13 was sequenced. The whole genome sequence comprises a circular chromosome and a circular plasmid. Cysteine desulfhydrase smCSE has been previously found to be associated with the synthesis of CdS quantum dots. Bioinformatics analysis indicated that the genome of sp. strain X13 encodes five putative cysteine desulfhydrases and all of them are located in the chromosome. The genome information may help us to determine the molecular mechanisms of the synthesis of CdS quantum dots and potentially enable us to engineer this microorganism for applications in biotechnology.
一种新的耐镉细菌，菌株X13，最近从重金属污染土壤中分离得到，该菌株能够利用硝酸镉[Cd(NO)]和L-半胱氨酸合成硫化镉量子点。菌株X13对硫化镉的生物矿化作用能够有效地从水溶液中去除镉。为了阐明硫化镉纳米颗粒生物合成的分子机制，对菌株X13的全基因组进行了测序。全基因组序列包括一条环状染色体和一个环状质粒。先前已发现半胱氨酸脱硫酶smCSE与硫化镉量子点的合成有关。生物信息学分析表明，菌株X13的基因组编码5种假定的半胱氨酸脱硫酶，它们均位于染色体上。基因组信息可能有助于我们确定硫化镉量子点合成的分子机制，并有可能使我们能够对这种微生物进行工程改造，以应用于生物技术领域。
