口腔鳞状细胞癌中 HIF-1 alpha 与 VEGF 的相关性：表达模式和定量免疫组化分析。

The correlation between HIF-1 alpha and VEGF in oral squamous cell carcinomas: Expression patterns and quantitative immunohistochemical analysis.

机构信息

Department of Oral and Maxillofacial Surgery, Taichung Veterans General Hospital, Taichung, Taiwan, ROC; Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan, ROC.

Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan, ROC; Oral and Maxillofacial Surgery, and Department of Dentistry, Chang Bing Show Chan Memorial Hospital, Changhwa, Taiwan, ROC.

出版信息

J Chin Med Assoc. 2018 Apr;81(4):370-375. doi: 10.1016/j.jcma.2017.06.025. Epub 2017 Dec 27.

DOI:10.1016/j.jcma.2017.06.025

PMID:29289482

Abstract

BACKGROUND

The prognostic predict biomarkers are important in oral squamous cell carcinoma (OSCC). We investigated the expression patterns and quantitation of hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in OSCC patients. Among OSCC patients with recurrence and metastasis, the expression percentages of HIF-1α and VEGF also were analyzed.

METHODS

Thirty-eight patients (8 hyperkeratosis, 30 oral squamous cell carcinoma) were included in this study. In the follow-up period, 5 OSCC patients had metastasis and 12 OSCC patients had local recurrence. We used computer-assisted image processing to analyze immunohistochemistry (IHC). The quantitative analysis of IHC slides, including upper-layer epithelium (U) and lower-layer epithelium (L), was calculated.

RESULTS

We found homogeneous expression of VEGF in the epithelium. However, two patterns of HIF-1α expression were observed: homogeneous and heterogeneous. The highest U + L layer percentage in HIF-1α and VEF expression had significant association in tumor metastasis and recurrence (p<0.001 in HIF-1α and p<0.001 in VEGF). U + L layer HIF-1α expression percentage was >156.4%, and the survival rate was poor (p < 0.001).

CONCLUSION

HIF-1α expression was not only influenced by tumor hypoxia, it also reflected tumor cell characteristics. High concentrations of VEGF and HIF-1α may have value as prognostic markers of tumor metastasis and recurrence.

摘要

背景

预后预测生物标志物在口腔鳞状细胞癌（OSCC）中很重要。我们研究了缺氧诱导因子-1α（HIF-1α）和血管内皮生长因子（VEGF）在 OSCC 患者中的表达模式和定量。在有复发和转移的 OSCC 患者中，还分析了 HIF-1α 和 VEGF 的表达百分比。

方法

本研究纳入 38 例患者（8 例角化过度，30 例口腔鳞状细胞癌）。在随访期间，5 例 OSCC 患者发生转移，12 例 OSCC 患者发生局部复发。我们使用计算机辅助图像处理来分析免疫组织化学（IHC）。对 IHC 切片进行定量分析，包括上皮的上层（U）和下层（L）。

结果

我们发现 VEGF 在上皮中呈均匀表达。然而，观察到 HIF-1α 表达有两种模式：均匀和不均匀。HIF-1α 和 VEF 表达的最高 U+L 层百分比与肿瘤转移和复发有显著相关性（HIF-1α 中 p<0.001，VEGF 中 p<0.001）。U+L 层 HIF-1α 表达百分比>156.4%，生存率较差（p<0.001）。