de Souza Diego M, de B Salum Lívia, Damasceno Robiedson R, de Moura Messias Pedro J, Silva Camila M, de S Cardoso João V, de O Morais Pedro A
Instituto de Criminalística, Polícia Civil do Distrito Federal, Brasília, DF, Brazil.
Instituto de Química, Universidade de Brasília, Brasília, DF, Brazil.
Anal Sci. 2024 Oct;40(10):1833-1841. doi: 10.1007/s44211-024-00617-4. Epub 2024 Jun 13.
The significance of accurate determination of ethanol content in hydrogel formulations was accentuated during COVID-19 pandemic coinciding with the heightened demand for sanitizing agents. The present article proposes three robust methodologies for this purpose: Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, and Densitometry with matrix effect correction by Near-Infrared Spectroscopy (NIR). All three methods demonstrated outstanding linearity (R ≥ 0.99) and minimal errors (< 1.7%), offering simplicity and operational efficiency. FTIR and Raman, being non-destructive and requiring minimal preparation, enable practical on-site analysis capabilities, underscoring the potential of the spectroscopic methods to expedite health investigations and inspections, empowering on-site ethanol determination, and relieving the burden on official laboratories. Additionally, the densitometry with NIR-based approach showcased superior accuracy and precision compared to spectroscopic methods, meeting validation criteria while offering operational advantages over the costly official distillation-based method. Therefore, it stands as a reliable and reproducible technique for comprehensive health and criminal compliance assessments, making it a compelling alternative for both industry and official laboratories.
在新冠疫情期间，随着对消毒剂需求的增加，准确测定水凝胶配方中乙醇含量的重要性愈发凸显。本文为此提出了三种可靠的方法：傅里叶变换红外光谱法（FTIR）、拉曼光谱法以及采用近红外光谱法（NIR）进行基质效应校正的密度测定法。这三种方法均表现出出色的线性（R≥0.99）和极小的误差（<1.7%），具备简便性和操作效率。FTIR和拉曼光谱法具有无损性且所需样品制备极少，具备实际现场分析能力，凸显了光谱法在加快健康调查和检查、实现现场乙醇测定以及减轻官方实验室负担方面的潜力。此外，基于近红外光谱法的密度测定法与光谱法相比，展现出更高的准确性和精密度，满足验证标准，同时在操作上比基于蒸馏的昂贵官方方法具有优势。因此，它是一种用于全面健康和刑事合规评估的可靠且可重复的技术，成为行业和官方实验室颇具吸引力的替代方法。
