Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, PR China.
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, PR China; Jiangxi Provincial Key Laboratory of Water Resources and Environment of Poyang Lake, Jiangxi Institute of Water Sciences, Nanchang 330029, PR China.
Chemosphere. 2018 Mar;195:817-824. doi: 10.1016/j.chemosphere.2017.12.058. Epub 2017 Dec 12.
In this study, an autotrophic denitrification process was successfully coupled with anammox to remove the nitrate by-product via electrochemical technology. When the voltage applied to the combined electrode reactor was 1.5 V, the electrode reaction removed nitrate by using the autotrophic denitrification biomass without affecting the anammox biomass. The nitrogen removal efficiency of the combined electrode reactor reached 99.1% without detectable nitrate at an influent NO-N/NH-N ratio of 1.5. On day 223, using the model calculations based on reaction equations, 19.7% of total nitrogen was removed via the autotrophic denitrification process, while the majority of nitrogen removal (approximately 79.4%) was attributed to the anammox reaction. Small variations of the population numbers and community structure of artificial bacteria according to electron microscopy predicted that the anammox and autotrophic denitrifying biomasses could coexist in the electrode reactor. Then, 16S rRNA analysis determined that the anammox biomass group was always dominant in mixed flora during continuous cultivation.
在这项研究中，通过电化学技术成功地将自养反硝化过程与厌氧氨氧化偶联，以去除硝酸盐副产物。当将 1.5V 的电压施加到组合电极反应器上时，电极反应利用自养反硝化生物量去除硝酸盐，而不会影响厌氧氨氧化生物量。在进水 NO-N/NH-N 比为 1.5 的情况下，组合电极反应器的氮去除效率达到 99.1%，而硝酸盐则无法检出。在第 223 天，根据反应方程式进行模型计算，19.7%的总氮通过自养反硝化过程去除，而大部分氮去除（约 79.4%）归因于厌氧氨氧化反应。根据电子显微镜预测的人工细菌种群数量和群落结构的微小变化表明，厌氧氨氧化和自养反硝化生物量可以在电极反应器中共存。然后，16S rRNA 分析确定，在连续培养过程中，厌氧氨氧化生物量组始终在混合菌群中占主导地位。
