Centre for Functional Food and Human Nutrition, School of Chemical and Life Sciences, Nanyang Polytechnic, Singapore; Food Science and Nutrition Group, School of Chemical and Life Sciences, Nanyang Polytechnic, Singapore.
Food Science and Nutrition Group, School of Chemical and Life Sciences, Nanyang Polytechnic, Singapore.
Nutrition. 2018 Feb;46:36-40. doi: 10.1016/j.nut.2017.08.010. Epub 2017 Sep 19.
The aim of this study was to examine the occurrence of five azo food dyes-tartrazine, sunset yellow, carmoisine, allura red, and ponceau 4 R-in the food supply chain of Singapore and their effects on the in vitro synthesis of leukotriene B (LTB) and F-isoprostanes.
Trained personnel recorded the names of foods and beverages sold in a local supermarket that contained at least one of the five azo dyes. The occurrence of the azo dyes in the local food supply was computed. The synthesis of LTB and F-isoprostanes from freshly isolated blood neutrophils were measured using gas chromatography-mass spectrometry.
Of the 1681 processed food items, 194 (11.54%) contained at least one of the five azo dyes. Tartrazine was most prevalent in food and beverage products sold in Singapore, followed by allura red, sunset yellow, ponceau 4 R, and carmoisine. The five azo dyes augmented the in vitro synthesis of LTB and F-isoprostanes from blood neutrophils. Tartrazine was significantly more potent in increasing LTB synthesis than the other dyes, which exhibited similar potencies. The five food dyes increased the formation of F-isoprostanes from blood neutrophils at all tested concentrations.
The high prevalence of azo dyes in the food supply of Singapore and their ability to elicit proinflammatory responses in vitro suggest a potential health risk to the local population.
本研究旨在检测新加坡食品供应链中五种偶氮食用染料（柠檬黄、日落黄、丽春红、诱惑红和胭脂红 4R）的存在情况，并研究其对体外白三烯 B（LTB）和 F-异前列烷合成的影响。
经过培训的人员记录了当地超市销售的至少含有五种偶氮染料之一的食品和饮料的名称。计算了当地食品供应中偶氮染料的存在情况。使用气相色谱-质谱法测量新鲜分离的血液中性粒细胞中 LTB 和 F-异前列烷的合成。
在 1681 种加工食品中，有 194 种（11.54%）至少含有五种偶氮染料之一。在新加坡销售的食品和饮料产品中，柠檬黄最为常见，其次是诱惑红、日落黄、胭脂红 4R 和丽春红。五种偶氮染料增强了血液中性粒细胞体外合成 LTB 和 F-异前列烷的能力。与其他具有相似效力的染料相比，柠檬黄在增加 LTB 合成方面的效力更为显著。五种食品染料在所有测试浓度下均增加了血液中性粒细胞中 F-异前列烷的形成。
偶氮染料在新加坡食品供应中的高流行率及其在体外引发炎症反应的能力表明，当地居民存在潜在的健康风险。
