Bembenek Bailey Stasia A, Niemuth Jennifer N, McClellan-Green Patricia D, Godfrey Matthew H, Harms Craig A, Stoskopf Michael K
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA.
Environmental Medicine Consortium, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, USA.
R Soc Open Sci. 2017 Nov 22;4(11):171433. doi: 10.1098/rsos.171433. eCollection 2017 Nov.
We used proton nuclear magnetic resonance spectroscopy (H-NMR) to evaluate metabolic impacts of environmentally relevant crude oil and Corexit exposures on the physiology of hatchling loggerhead sea turtles (). Sample extraction and data acquisition methods for very small volume whole blood samples and sources of variation between individual hatchlings were assessed. Sixteen unclotted, whole blood samples were obtained from 7-day-old hatchlings after a 4-day cutaneous exposure to either control seawater, crude oil, Corexit 9500A or a combination of crude oil and Corexit 9500A. After extraction, one- and two-dimensional H-NMR spectra of the samples were obtained, and 17 metabolites were identified and confirmed in the whole blood spectra. Variation among samples due to the concentrations of metabolites 3-hydroxybutyrate, lactate, trimethylamine oxide and propylene glycol did not statistically correlate with treatment group. However, the characterization of the hatchling loggerhead whole blood metabolome provides a foundation for future metabolomic research with sea turtles and a basis for the study of tissues from exposed hatchling sea turtles.
我们使用质子核磁共振波谱法（H-NMR）来评估环境相关原油和Corexit暴露对蠵龟幼龟生理的代谢影响。评估了极少量全血样本的样品提取和数据采集方法以及个体幼龟之间的变异来源。在对7日龄幼龟进行为期4天的皮肤暴露于对照海水、原油、Corexit 9500A或原油与Corexit 9500A的组合后，采集了16份未凝血的全血样本。提取后，获得样品的一维和二维H-NMR光谱，并在全血光谱中鉴定和确认了17种代谢物。代谢物3-羟基丁酸、乳酸、氧化三甲胺和丙二醇的浓度导致的样本间变异与治疗组无统计学相关性。然而，蠵龟幼龟全血代谢组的表征为未来海龟代谢组学研究提供了基础，并为研究暴露的蠵龟幼龟组织提供了依据。
