• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

癫痫动物模型海马齿状回中失调 microRNAs 的荟萃分析。

Meta-Analysis of MicroRNAs Dysregulated in the Hippocampal Dentate Gyrus of Animal Models of Epilepsy.

机构信息

Division of Brain Sciences, Imperial College London, Charing Cross Hospital, W12 0NN London, United Kingdom.

Division of Neuroscience, University Vita-Salute San Raffaele, Milan 20132, Italy.

出版信息

eNeuro. 2017 Dec 28;4(6). doi: 10.1523/ENEURO.0152-17.2017. eCollection 2017 Nov-Dec.

DOI:10.1523/ENEURO.0152-17.2017
PMID:29291240
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5745610/
Abstract

The identification of mechanisms transforming normal to seizure-generating tissue after brain injury is key to developing new antiepileptogenic treatments. MicroRNAs (miRNAs) may act as regulators and potential treatment targets for epileptogenesis. Here, we undertook a meta-analysis of changes in miRNA expression in the hippocampal dentate gyrus (DG) following an epileptogenic insult in three epilepsy models. We identified 26 miRNAs significantly differentially expressed during epileptogenesis, and five differentially expressed in chronic epilepsy. Of these, 13 were not identified in any of the individual studies. To assess the role of these miRNAs, we predicted their mRNA targets and then filtered the list to include only target genes expressed in DG and negatively correlated with miRNA expression. Functional enrichment analysis of mRNA targets of miRNAs dysregulated during epileptogenesis suggested a role for molecular processes related to inflammation and synaptic function. Our results identify new miRNAs associated with epileptogenesis from existing data, highlighting the utility of meta-analysis in maximizing value from preclinical data.

摘要

在脑损伤后识别将正常组织转化为致痫组织的机制是开发新的抗癫痫发生治疗方法的关键。 microRNAs (miRNAs) 可能作为癫痫发生的调节剂和潜在治疗靶点。在这里，我们对三种癫痫模型中海马齿状回 (DG) 中 miRNA 表达的变化进行了荟萃分析。我们鉴定了在癫痫发生过程中显著差异表达的 26 个 miRNA，以及在慢性癫痫中差异表达的 5 个 miRNA。其中，有 13 个 miRNA 在任何一个单独的研究中都没有被发现。为了评估这些 miRNA 的作用，我们预测了它们的 mRNA 靶标，然后过滤掉只包括在 DG 中表达且与 miRNA 表达呈负相关的靶基因的列表。对癫痫发生过程中失调的 miRNA 的 mRNA 靶标的功能富集分析表明，与炎症和突触功能相关的分子过程可能起作用。我们的研究结果从现有数据中确定了与癫痫发生相关的新 miRNA，突出了荟萃分析在从临床前数据中最大化价值的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a76/5745610/efb4a8174ecb/enu0061724880005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a76/5745610/cbcd5309a2ea/enu0061724880001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a76/5745610/ac8f33730b7d/enu0061724880002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a76/5745610/78bbadfffaa3/enu0061724880003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a76/5745610/545b3bfc688a/enu0061724880004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a76/5745610/efb4a8174ecb/enu0061724880005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a76/5745610/cbcd5309a2ea/enu0061724880001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a76/5745610/ac8f33730b7d/enu0061724880002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a76/5745610/78bbadfffaa3/enu0061724880003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a76/5745610/545b3bfc688a/enu0061724880004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a76/5745610/efb4a8174ecb/enu0061724880005.jpg

相似文献

1
Meta-Analysis of MicroRNAs Dysregulated in the Hippocampal Dentate Gyrus of Animal Models of Epilepsy.癫痫动物模型海马齿状回中失调 microRNAs 的荟萃分析。
eNeuro. 2017 Dec 28;4(6). doi: 10.1523/ENEURO.0152-17.2017. eCollection 2017 Nov-Dec.
2
Hippocampal subregion-specific microRNA expression during epileptogenesis in experimental temporal lobe epilepsy.在实验性颞叶癫痫发生期间海马亚区特异性 microRNA 表达。
Neurobiol Dis. 2014 Feb;62:508-20. doi: 10.1016/j.nbd.2013.10.026. Epub 2013 Oct 31.
3
MicroRNA profiling in the dentate gyrus in epileptic rats: The role of miR-187-3p.癫痫大鼠齿状回中的微小RNA谱分析：miR-187-3p的作用
Medicine (Baltimore). 2017 Jun;96(22):e6744. doi: 10.1097/MD.0000000000006744.
4
MicroRNAs and target genes in epileptogenesis.微小 RNA 与癫痫发生中的靶基因。
Epilepsia. 2020 Oct;61(10):2086-2096. doi: 10.1111/epi.16687. Epub 2020 Sep 18.
5
Neuronal MeCP2 in the dentate gyrus regulates mossy fiber sprouting of mice with temporal lobe epilepsy.齿状回神经元 MeCP2 调节颞叶癫痫小鼠的苔藓纤维发芽。
Neurobiol Dis. 2023 Nov;188:106346. doi: 10.1016/j.nbd.2023.106346. Epub 2023 Nov 4.
6
MicroRNAs in epilepsy: pathophysiology and clinical utility.癫痫中的 microRNAs：病理生理学和临床应用。
Lancet Neurol. 2016 Dec;15(13):1368-1376. doi: 10.1016/S1474-4422(16)30246-0.
7
A role of dentate gyrus mechanistic target of rapamycin activation in epileptogenesis in a mouse model of posttraumatic epilepsy.齿状回雷帕霉素靶蛋白激活在创伤后癫痫小鼠模型中的致痫作用。
Epilepsia. 2024 Jul;65(7):2127-2137. doi: 10.1111/epi.18011. Epub 2024 May 18.
8
Integrative network analysis of miRNA-mRNA expression profiles during epileptogenesis in rats reveals therapeutic targets after emergence of first spontaneous seizure.在大鼠癫痫发生过程中 miRNA-mRNA 表达谱的综合网络分析揭示了首次自发性发作后治疗靶点的出现。
Sci Rep. 2024 Jul 3;14(1):15313. doi: 10.1038/s41598-024-66117-7.
9
Hippocampal sub-regional differences in the microRNA response to forebrain ischemia.海马亚区在前脑缺血后 microRNA 反应的差异。
Mol Cell Neurosci. 2019 Jul;98:164-178. doi: 10.1016/j.mcn.2019.05.003. Epub 2019 May 23.
10
Increased mitotic activity in the dentate gyrus of the hippocampus of adult C57BL/6J mice exposed to the flurothyl kindling model of epileptogenesis.在暴露于氟代乙酰胺点燃癫痫模型的成年C57BL/6J小鼠海马齿状回中，有丝分裂活性增加。
Neuroscience. 2002;115(3):669-83. doi: 10.1016/s0306-4522(02)00514-6.

引用本文的文献

1
Identification of plasma exosomal microRNAs and bioinformatics analysis of the microRNA-messenger RNA regulatory pathways in mice with status epilepticus.癫痫持续状态小鼠血浆外泌体微小RNA的鉴定及微小RNA-信使核糖核酸调控通路的生物信息学分析
Cytotechnology. 2025 Apr;77(2):65. doi: 10.1007/s10616-025-00708-8. Epub 2025 Feb 20.
2
Integrative network analysis of miRNA-mRNA expression profiles during epileptogenesis in rats reveals therapeutic targets after emergence of first spontaneous seizure.在大鼠癫痫发生过程中 miRNA-mRNA 表达谱的综合网络分析揭示了首次自发性发作后治疗靶点的出现。
Sci Rep. 2024 Jul 3;14(1):15313. doi: 10.1038/s41598-024-66117-7.
3

本文引用的文献

1
Is autopsy tissue a valid control for epilepsy surgery tissue in microRNA studies?在微小RNA研究中，尸检组织能否作为癫痫手术组织的有效对照？
Epilepsia Open. 2016 Nov 9;2(1):90-95. doi: 10.1002/epi4.12023. eCollection 2017 Mar.
2
A microRNA-129-5p/Rbfox crosstalk coordinates homeostatic downscaling of excitatory synapses.微小RNA-129-5p与Rbfox相互作用协调兴奋性突触的稳态下调。
EMBO J. 2017 Jun 14;36(12):1770-1787. doi: 10.15252/embj.201695748. Epub 2017 May 9.
3
Transcriptional profile of hippocampal dentate granule cells in four rat epilepsy models.
MicroRNA-335-5p suppresses voltage-gated sodium channel expression and may be a target for seizure control.
微小 RNA-335-5p 抑制电压门控钠离子通道表达，可能是控制癫痫发作的靶点。
Proc Natl Acad Sci U S A. 2023 Jul 25;120(30):e2216658120. doi: 10.1073/pnas.2216658120. Epub 2023 Jul 18.
4
Age-Related microRNA Overexpression in Lafora Disease Male Mice Provides Links between Neuroinflammation and Oxidative Stress.年龄相关 microRNA 在拉佛拉病雄性小鼠中的过度表达提供了神经炎症和氧化应激之间的联系。
Int J Mol Sci. 2023 Jan 6;24(2):1089. doi: 10.3390/ijms24021089.
5
An Insight into the microRNAs Associated with Arteriovenous and Cavernous Malformations of the Brain.对与脑动静脉畸形和海绵状畸形相关的微小RNA的深入研究。
Cells. 2021 Jun 2;10(6):1373. doi: 10.3390/cells10061373.
6
Genetic deletion of microRNA-22 blunts the inflammatory transcriptional response to status epilepticus and exacerbates epilepsy in mice.基因敲除 microRNA-22 可减轻癫痫持续状态引起的炎症转录反应，并加重小鼠癫痫发作。
Mol Brain. 2020 Aug 21;13(1):114. doi: 10.1186/s13041-020-00653-x.
7
A systems approach delivers a functional microRNA catalog and expanded targets for seizure suppression in temporal lobe epilepsy.系统方法提供了一个功能性 microRNA 目录，并为颞叶癫痫的癫痫抑制扩大了靶标。
Proc Natl Acad Sci U S A. 2020 Jul 7;117(27):15977-15988. doi: 10.1073/pnas.1919313117. Epub 2020 Jun 24.
8
micro-RNAs dependent regulation of DNMT and HIF1α gene expression in thrombotic disorders.miRNA 依赖性调控在血栓性疾病中 DNMT 和 HIF1α 基因的表达。
Sci Rep. 2019 Mar 20;9(1):4815. doi: 10.1038/s41598-018-38057-6.
9
Discovery and validation of blood microRNAs as molecular biomarkers of epilepsy: Ways to close current knowledge gaps.血液中微小RNA作为癫痫分子生物标志物的发现与验证：弥补当前知识空白的方法
Epilepsia Open. 2018 Oct 30;3(4):427-436. doi: 10.1002/epi4.12275. eCollection 2018 Dec.
10
Regulation of Ion Channels by MicroRNAs and the Implication for Epilepsy.MicroRNAs 对离子通道的调控及其在癫痫中的意义。
Curr Neurol Neurosci Rep. 2018 Jul 25;18(9):60. doi: 10.1007/s11910-018-0870-2.
四个大鼠癫痫模型中海马齿状回颗粒细胞的转录谱。
Sci Data. 2017 May 9;4:170061. doi: 10.1038/sdata.2017.61.
4
The C1q complement family of synaptic organizers: not just complementary.C1q 补体家族的突触组织者：不只是互补。
Curr Opin Neurobiol. 2017 Aug;45:9-15. doi: 10.1016/j.conb.2017.02.002. Epub 2017 Feb 20.
5
MicroRNAs in epilepsy: pathophysiology and clinical utility.癫痫中的 microRNAs：病理生理学和临床应用。
Lancet Neurol. 2016 Dec;15(13):1368-1376. doi: 10.1016/S1474-4422(16)30246-0.
6
MicroRNA in Control of Gene Expression: An Overview of Nuclear Functions.控制基因表达的微小RNA：核功能概述
Int J Mol Sci. 2016 Oct 13;17(10):1712. doi: 10.3390/ijms17101712.
7
Analysis of Post-Traumatic Brain Injury Gene Expression Signature Reveals Tubulins, Nfe2l2, Nfkb, Cd44, and S100a4 as Treatment Targets.创伤性脑损伤后基因表达特征分析显示微管蛋白、Nfe2l2、Nfkb、Cd44 和 S100a4 可作为治疗靶点。
Sci Rep. 2016 Aug 17;6:31570. doi: 10.1038/srep31570.
8
Involvement of microRNAs in epileptogenesis.微小RNA在癫痫发生中的作用。
Epilepsia. 2016 Jul;57(7):1015-26. doi: 10.1111/epi.13404. Epub 2016 May 21.
9
Dual and Opposing Roles of MicroRNA-124 in Epilepsy Are Mediated through Inflammatory and NRSF-Dependent Gene Networks.微小RNA-124在癫痫中的双重和相反作用是通过炎症和NRSF依赖性基因网络介导的。
Cell Rep. 2016 Mar 15;14(10):2402-12. doi: 10.1016/j.celrep.2016.02.042. Epub 2016 Mar 3.
10
NFκB-inducing kinase inhibits NFκB activity specifically in neurons of the CNS.核因子κB诱导激酶特异性抑制中枢神经系统神经元中的核因子κB活性。
J Neurochem. 2016 Apr;137(2):154-63. doi: 10.1111/jnc.13526. Epub 2016 Mar 15.