淋病奈瑟菌的保存：拭子是否应冷藏？：淋病奈瑟菌的保存

Preservation of Neisseria gonorrhoeae: should swabs be refrigerated or not?: Neisseria gonorrhoeae preservation.

作者信息

Serra-Pladevall Judit, Gulin Blanco Carlos, Vila Olmo Neus, Arjona Camacho Pilar, Andreu Domingo Antonia

机构信息

Microbiology Department, Hospital Universitari Vall d'Hebron, Pg. Vall d'Hebron, 119-129. 08035, Barcelona, Spain; Universitat Autonoma de Barcelona, Plaça Cívica, s/n, 08193 Bellaterra, Barcelona, Spain.

Microbiology Department, Hospital Universitari Vall d'Hebron, Pg. Vall d'Hebron, 119-129. 08035, Barcelona, Spain.

出版信息

J Microbiol Methods. 2018 Feb;145:37-39. doi: 10.1016/j.mimet.2017.12.012. Epub 2017 Dec 29.

DOI:10.1016/j.mimet.2017.12.012

PMID:29292202

Abstract

Although molecular diagnosis has replaced culture as the reference technique in gonococcal infection, conventional culture is still performed. The aim was to compare the survival of Neisseria gonorrhoeae (NG) in transport swabs at room temperature (RT) and at 4°C. Fifty-four NG isolates were tested. A 1.5×10CFUmL suspension was prepared for each strain, in which two swabs (Amies liquid medium with flocked swabs) were absorbed; one was preserved at RT and the other at 4°C. At 24 and 48h, 150μL of Amies medium were plated in Thayer Martin agar. The recovery percentage was significantly higher for the swabs kept at 4°C than for those kept at RT, both at 24h (94.4% vs. 16.7%), and at 48h (37% vs. 0%). This study clearly demonstrates the higher survival of NG when transport swabs are kept refrigerated. Modifying the guidelines could greatly improve the efficiency of microbiological diagnosis of the gonococcal infection.

摘要

尽管分子诊断已取代培养成为淋病奈瑟菌感染的参考技术，但仍会进行传统培养。目的是比较淋病奈瑟菌（NG）在室温（RT）和4°C的运输拭子中的存活情况。测试了54株NG分离株。为每个菌株制备1.5×10CFU/mL的悬浮液，其中两根拭子（含脱脂棉拭子的阿氏液体培养基）被浸入；一根保存在室温下，另一根保存在4°C。在24小时和48小时时，将150μL阿氏培养基接种在泰-马二氏琼脂上。在24小时（94.4%对16.7%）和48小时（37%对0%）时，保存在4°C的拭子的回收率明显高于保存在室温下的拭子。这项研究清楚地表明，运输拭子冷藏时NG的存活率更高。修改指南可大大提高淋病感染微生物诊断的效率。