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用于淋病奈瑟菌培养的市售阿氏运送系统与自制阿氏培养基的比较。

Comparison of commercial Amies transport systems with in-house Amies medium for recovery of Neisseria gonorrhoeae.

作者信息

Thompson D S, French S A

机构信息

Orillia Public Health Laboratory, Ministry of Health, Ontario, Canada L3V 6K5.

出版信息

J Clin Microbiol. 1999 Sep;37(9):3020-1. doi: 10.1128/JCM.37.9.3020-3021.1999.

Abstract

Microbiologists are still encumbered by the variable performance of Amies charcoal transport medium in recovery of Neisseria gonorrhoeae. The objective of this study was to evaluate and select a good quality commercial system to replace our in-house preparation. We adsorbed 0.1 ml of a suspension from 30 gonococcal isolates onto each swab type and replaced the swab into the transport medium. We plated the swabs to New York City medium at 0, 24, 48, 72, and 96 h. We compared the survival of each isolate in the commercial Amies transport systems with that in our in-house Amies transport medium. The best recovery was observed with Copan transport systems. Some systems are inadequate and unacceptable for culture of gonococci.

摘要

微生物学家们仍受限于用于淋病奈瑟菌培养的阿氏炭末运送培养基的不同表现。本研究的目的是评估并挑选一种高质量的商业系统来取代我们的自制培养基。我们将来自30株淋球菌分离株的0.1 ml菌悬液吸附到每种拭子类型上,然后将拭子放回运送培养基中。我们分别在0、24、48、72和96小时将拭子接种到纽约市培养基上。我们比较了每种分离株在商业阿氏运送系统和我们自制的阿氏运送培养基中的存活情况。Copan运送系统的回收率最高。有些系统对于淋球菌培养来说是不充分且不可接受的。

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