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快速育种是加速作物研究和育种的有力工具。

Speed breeding is a powerful tool to accelerate crop research and breeding.

机构信息

Queensland Alliance for Agriculture and Food Innovation, University of Queensland, St Lucia, Queensland, Australia.

John Innes Centre, Norwich Research Park, Norwich, UK.

出版信息

Nat Plants. 2018 Jan;4(1):23-29. doi: 10.1038/s41477-017-0083-8. Epub 2018 Jan 1.

Abstract

The growing human population and a changing environment have raised significant concern for global food security, with the current improvement rate of several important crops inadequate to meet future demand . This slow improvement rate is attributed partly to the long generation times of crop plants. Here, we present a method called 'speed breeding', which greatly shortens generation time and accelerates breeding and research programmes. Speed breeding can be used to achieve up to 6 generations per year for spring wheat (Triticum aestivum), durum wheat (T. durum), barley (Hordeum vulgare), chickpea (Cicer arietinum) and pea (Pisum sativum), and 4 generations for canola (Brassica napus), instead of 2-3 under normal glasshouse conditions. We demonstrate that speed breeding in fully enclosed, controlled-environment growth chambers can accelerate plant development for research purposes, including phenotyping of adult plant traits, mutant studies and transformation. The use of supplemental lighting in a glasshouse environment allows rapid generation cycling through single seed descent (SSD) and potential for adaptation to larger-scale crop improvement programs. Cost saving through light-emitting diode (LED) supplemental lighting is also outlined. We envisage great potential for integrating speed breeding with other modern crop breeding technologies, including high-throughput genotyping, genome editing and genomic selection, accelerating the rate of crop improvement.

摘要

人口增长和环境变化引起了人们对全球粮食安全的极大关注,目前几种重要作物的改良速度不足以满足未来的需求。这种缓慢的改良速度部分归因于作物的长世代时间。在这里,我们提出了一种称为“快速繁殖”的方法,它大大缩短了世代时间,加速了育种和研究计划。快速繁殖可使春小麦(Triticum aestivum)、硬粒小麦(T. durum)、大麦(Hordeum vulgare)、鹰嘴豆(Cicer arietinum)和豌豆(Pisum sativum)每年达到 6 代,油菜(Brassica napus)达到 4 代,而不是在正常温室条件下的 2-3 代。我们证明,在完全封闭、受控环境生长室中的快速繁殖可以加速植物的研究发展,包括对成年植物性状的表型分析、突变体研究和转化。在温室环境中使用补充照明可以通过单粒下降(SSD)快速进行世代循环,并有可能适应更大规模的作物改良计划。通过使用发光二极管(LED)补充照明也可以节省成本。我们设想将快速繁殖与其他现代作物育种技术(包括高通量基因分型、基因组编辑和基因组选择)相结合具有很大的潜力,可以加速作物改良的速度。

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