School of Agriculture and Food Sciences, University of Queensland, Brisbane, 4072, Australia.
Theor Appl Genet. 2015 Jun;128(6):1039-47. doi: 10.1007/s00122-015-2488-y. Epub 2015 Mar 10.
We characterise the distribution of crossover and non-crossover recombination in Brassica napus and Cicer arietinum using a low-coverage genotyping by sequencing pipeline SkimGBS. The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.
我们使用低覆盖度测序分型（SkimGBS）方法来描绘甘蓝型油菜和鹰嘴豆中交叉重组和非交叉重组（基因转换）的分布。新一代 DNA 测序技术的发展使得基于序列的基因分型在多样性评估、基因组结构验证和基因-性状关联等应用中得到了快速应用。我们已经为作物植物建立了一种基于 skim 的测序分型方法，并将该方法应用于甘蓝型油菜和鹰嘴豆的基因型分离群体中。将后代基因型与亲本个体的基因型进行比较，可以鉴定交叉重组和非交叉重组（基因转换）事件。我们的结果以高分辨率确定了重组事件的位置，从而可以对分离群体中的重组进行作图和频率评估。
