Wen Jiangfeng, Li Yang, Qi Tiancong, Gao Hua, Liu Bei, Zhang Min, Huang Huang, Song Susheng
a School of Life Sciences, Tsinghua University , Beijing , China.
b Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement , College of Life Sciences, Capital Normal University , Beijing , China.
Plant Signal Behav. 2018 Jan 2;13(1):e1422460. doi: 10.1080/15592324.2017.1422460. Epub 2018 Jan 16.
The phytohormone jasmonates (JAs) regulate plant development, growth, secondary metabolism, and defense responses. JAs act through CORONATINE INSENSITIVE1 (COI1) to induce the degradation of JA ZIM-domain (JAZ) proteins, and activate JAZ-repressed transcription factors to regulate plant response. We previously showed that the basic helix-loop-helix (bHLH) and MYB members of the WD-repeat/bHLH/MYB complex interacted with JAZs and mediated JA-induced anthocyanin accumulation and trichome initiation. In this study, we showed that the C-terminal domain of the bHLH members (GLABRA3 [GL3], ENHANCER OF GLABRA3 [EGL3] and TRANSPARENT TESTA8 [TT8]) interacted with JAZs in yeast and plant, and mediated dimerizations between the bHLH members. Our study provides further understanding of the bHLH members of the WD-repeat/bHLH/MYB complex in JA pathway.
植物激素茉莉酸（JAs）调控植物的发育、生长、次生代谢和防御反应。JAs通过茉莉酸不敏感蛋白1（COI1）发挥作用，诱导茉莉酸锌指结构域（JAZ）蛋白降解，并激活受JAZ抑制的转录因子来调控植物反应。我们之前的研究表明，WD重复/碱性螺旋-环-螺旋/ MYB复合物中的碱性螺旋-环-螺旋（bHLH）成员和MYB成员与JAZ相互作用，介导茉莉酸诱导的花青素积累和毛状体起始。在本研究中，我们发现bHLH成员（光叶无毛3 [GL3]、光叶无毛3增强子[EGL3]和透明种皮8 [TT8]）的C末端结构域在酵母和植物中与JAZ相互作用，并介导bHLH成员之间的二聚化。我们的研究进一步加深了对WD重复/bHLH/MYB复合物中bHLH成员在茉莉酸信号通路中的理解。
