MOE Key Laboratory of Protein Science, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Mol Plant. 2011 Mar;4(2):279-88. doi: 10.1093/mp/ssq073. Epub 2011 Jan 17.
The Arabidopsis Jasmonate ZIM-domain proteins (JAZs) act as substrates of SCF(COI1) complex to repress their downstream targets, which are essential for JA-regulated plant development and defense. The bHLH transcription factor MYC2 was found to interact with JAZs and mediate JA responses including JA-inhibitory root growth. Here, we identified another bHLH transcription factor MYC3 which directly interacted with JAZs by virtue of its N-terminal region to regulate JA responses. The transgenic plants with overexpression of MYC3 exhibited hypersensitivity in JA-inhibitory root elongation and seedling development. The JAZ-interacting pattern and the JA-induced expression pattern of MYC3 were distinguishable from those of MYC2. We speculate that MYC3 and MYC2 may have redundant but also distinguishable functions in regulation of JA responses.
拟南芥茉莉酸 ZIM 结构域蛋白（JAZs）作为 SCF（COI1）复合物的底物发挥作用，抑制其下游靶标，这些靶标对茉莉酸调节的植物发育和防御至关重要。bHLH 转录因子 MYC2 被发现与 JAZs 相互作用，并介导包括茉莉酸抑制根生长在内的茉莉酸反应。在这里，我们鉴定了另一个 bHLH 转录因子 MYC3，它通过其 N 端区域直接与 JAZs 相互作用，从而调节茉莉酸反应。过表达 MYC3 的转基因植物在茉莉酸抑制根伸长和幼苗发育方面表现出超敏性。MYC3 的 JAZ 相互作用模式和茉莉酸诱导的表达模式与 MYC2 不同。我们推测 MYC3 和 MYC2 可能在茉莉酸反应的调节中具有冗余但也可区分的功能。
