Morgan R J, Gonchoroff N J, Katzmann J A, Witzig T E, Kyle R A, Greipp P R
Mayo Graduate School of Medicine, Rochester, Minnesota 55905.
Am J Hematol. 1989 Apr;30(4):195-200. doi: 10.1002/ajh.2830300402.
We analyzed 49 patients with multiple myeloma (MM) using propidium iodide (PI) staining and flow cytometry to assess DNA content of MM cells in bone marrow. In 12 patients the presence or absence of DNA content aneuploidy could not be determined by analysis of single-parameter DNA histograms alone. Using forward light scatter measurement, resolution of ploidy was readily obtained in all but three cases. Assuming the DNA content of the low light scatter population to be diploid, we were able to classify 7 cases as hypodiploid and 39 as hyperdiploid or diploid. Hyperdiploid or diploid patients survived a median of 24 months and hypodiploid patients only 2.5 months. We conclude that multiparameter analysis of DNA content and light scatter provides easier analysis for the presence or absence of aneuploidy. Patients with DNA content hypodiploidy have a short survival.
我们使用碘化丙啶（PI）染色和流式细胞术分析了49例多发性骨髓瘤（MM）患者，以评估骨髓中MM细胞的DNA含量。在12例患者中，仅通过单参数DNA直方图分析无法确定DNA含量非整倍体的存在与否。使用前向光散射测量，除3例病例外，在所有病例中都很容易获得倍性分辨率。假设低光散射群体的DNA含量为二倍体，我们能够将7例分类为亚二倍体，39例分类为超二倍体或二倍体。超二倍体或二倍体患者的中位生存期为24个月，而亚二倍体患者仅为2.5个月。我们得出结论，对DNA含量和光散射进行多参数分析可为非整倍体的存在与否提供更简便的分析。DNA含量为亚二倍体的患者生存期较短。
