纳米结构超支化β-环糊精上的RNA负载
RNA Loading on Nano-Structured Hyperbranched β-Cyclodextrin.
作者信息
Hirbod Sorina, Nafisi Shohreh, Maibach Howard I
机构信息
Department of Chemistry, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Department of Dermatology, University of California, San Francisco, CA, USA.
出版信息
Avicenna J Med Biotechnol. 2018 Jan-Mar;10(1):15-21.
BACKGROUND
β-Cyclodextrin functionalized hyper-branched polyglycerol (HBCD: β-CD-g-PG), a biocompatible polymer, has recently been proposed for delivery of poorly water soluble compounds.
METHODS
The present study examines the interaction of HBCD with RNA, utilizing a constant concentration of RNA and different HBCD/RNA ratios of 1/16 to 1/1, at physiological condition in an aqueous solution. Circular Dichroism (CD), UV-visible, FTIR spectroscopic methods, zeta potential and Dynamic Light Scattering (DLS) were used to analyze the particle formation, particle charge, particle size, aggregation, RNA conformation, binding constant and mode, and the effect of polymer complexation on RNA stability.
RESULTS
The results indicate that the interaction of RNA with HBCD leads to the formation of a linear dendritic supramolecule biopolymer with an overall binding constant of K= 1.25 × 10.
CONCLUSION
The small sized synthesized polymer can be considered as an appropriate system for preventing RNA aggregation and protecting the gene by host-guest interaction.
背景
β-环糊精功能化超支化聚甘油（HBCD：β-CD-g-PG），一种生物相容性聚合物，最近被提议用于递送难溶性化合物。
方法
本研究在生理条件下的水溶液中，利用恒定浓度的RNA和1/16至1/1的不同HBCD/RNA比例，研究了HBCD与RNA的相互作用。采用圆二色性（CD）、紫外可见、傅里叶变换红外光谱法、zeta电位和动态光散射（DLS）来分析颗粒形成、颗粒电荷、颗粒大小、聚集、RNA构象、结合常数和模式，以及聚合物络合对RNA稳定性的影响。
结果
结果表明，RNA与HBCD的相互作用导致形成线性树枝状超分子生物聚合物，其总结合常数为K = 1.25 × 10。
结论
合成的小尺寸聚合物可被视为通过主客体相互作用防止RNA聚集和保护基因的合适体系。
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